Mouse CASR (Calcium Sensing Receptor) ELISA Kit

• Description:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CASR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CASR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CASR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CASR in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative:

  CAR; FHH; FIH; GPRC2A; HHC; HHC1; NSHPT; PCAR1; Parathyroid cell calcium-sensing receptor 1; Hypocalciuric Hypercalcemia 1; Severe Neonatal Hyperparathyroidism

• Gene Aliases:

  CAR; FHH; FIH; GPRC2A; HHC; HHC1; NSHPT; PCAR1; Parathyroid cell calcium-sensing receptor 1; Hypocalciuric Hypercalcemia 1; Severe Neonatal Hyperparathyroidism

• UniProt:

  Q9QY96

• Reactivity:

  Mouse

• Field of Research:

  Metabolic pathway, Endocrinology, Neuro science, Hormone metabolism

• Assay Type:

  Sandwich

• Assay Principle:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CASR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CASR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CASR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CASR in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  3.5 H

• Standard:

  10 ng/mL

• Sample Type:

  Tissue homogenates, cell lysates and other biological fluids.

• Detection Range:

  0.16 - 10 ng/mL

• Sensitivity:

  0.058 ng/mL

• Species:

  Mouse

• CAS Number:

  7732-18-5