Anti-CD5(Cris-1)

• Description:

  Recognizes a 67 kDa transmembrane protein, which is identified as CD5 (HLDA I; WS Code T 29HLDA III; WS Code T 530). The CD5 antigen is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. Anti-CD5 is a pan T-cell marker that also reacts with a range of neoplastic B-cells, e.g. chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), mantle cell lymphoma, and a subset (~10%) of diffuse large B-cell lymphoma. CD5 aberrant expression is useful in making a diagnosis of mature T-cell neoplasms.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  CD5 antigen (p56 62), LEU1, Ly12, LyA, Lymphocyte antigen T1/Leu-1, Lymphocyte glycoprotein T1/Leu1, T-cell surface glycoprotein CD5

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  CD5

• Gene ID:

  921

• NCBI Gene ID:

  58685

• UniProt:

  P06127

• Cellular Locus:

  Plasma membrane

• Host:

  Mouse

• Species Reactivity:

  Human, Rhesus macaque

• Immunogen:

  Stimulated human leukocytes

• Target Antigen:

  CD5

• Clonality:

  Monoclonal

• Isotype:

  IgG2a κ

• Clone:

  Cris-1

• Conjugation:

  Purified, BSA-free

• Disease:

  Tumor

• Source:

  Animal

• Applications:

  Flow (verified) | ELISA (published) | Functional studies (published) | IP (published)

• Validated Applications:

  FC, ELISA, IP

• Field of Research:

  Immunology

• Positive Control:

  293T, Ramos, or MOLT-4 Cells. Tonsil.

• Concentration:

  1 mg/mL

• Buffer:

  PBS, no BSA, no azide

• Molecular Weight:

  67 kDa

• Additionnal Information:

  Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Immunofluorescence 0.5-1 ug/mL Immunoprecipitation 0.5-1 ug/500 ug protein lysate|Optimal dilution for a specific application should be determined by user

• References & Citations:

  Note: References for this clone sold by other suppliers may be listed for expected applications. Immunology (1990) 70, 434-439. (Flow) J Immunol (1992) 148(5):1287-93. (receptor activation) J Immunol (1993) 151 (9): 4423-4430. (IP) Nature (1991) 351: 662-665. (ELISA)

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  -35°C to -5°C ; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4