Anti-CD11b(M1/70), CF647 conjugate

• Description:

  CD11b is a cell adhesion molecule that acts as a receptor for cell surface ligands such as intracellular adhesion molecules (ICAMs) or soluble ligands. Integrins are heterodimeric proteins that contain an alpha chain and beta chain. IntegrinαM combines with the Integrinβ2 to form a leukocyte-specific integrin referred to as macrophage receptor 1 (Mac-1), or inactivated-C3b (iC3b) receptor 3 (CR3). IntegrinαM/β2 is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. The protein CD11b has been implicated in the various adhesion-related interactions of cells such as monocytes, macrophages, natural killer (NK) cells, and granulocytes. It is part of a heterodimer that consists of CD11b and CD18. It also modulates the uptake of complement-coated particles within the cell. It is commonly used as a microglial marker in tissues derived from the nervous system.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  CD11 antigen-like family member B|CD11b/CD18|CD49d|Cell surface glycoprotein MAC-1 subunit alpha|Complement Component Receptor 3 Alpha|CR3 Alpha Chain (CR3A)|Integrin alpha-M|Integrin beta 2 alpha subunit|ITGAM|Leukocyte adhesion receptor MO1; Ly-40|Mac-1a|MAC1|Mac1, alpha subunit|MAC1A|Macrophage antigen alpha polypeptide; MO1A|Neutrophil adherence receptor alpha M subunit

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  ITGAM

• Gene ID:

  3684

• NCBI Gene ID:

  172631

• UniProt:

  P11215

• Cellular Locus:

  Plasma membrane

• Host:

  Rat

• Species Reactivity:

  Baboon, Chimpanzee, Human, Mouse, Rabbit, Rhesus macaque

• Immunogen:

  B10 mouse spleen cells enriched for T lymphocytes

• Target Antigen:

  CD11b | MAC-1

• Clonality:

  Monoclonal

• Isotype:

  IgG2b κ

• Clone:

  M1/70

• Conjugation:

  CF647

• Source:

  Animal

• Applications:

  Flow, surface (published) | Functional studies (published) | IF (published) | IP (published)

• Validated Applications:

  FC, IF, IP

• Field of Research:

  Immunology, Neuroscience

• Positive Control:

  Monocytes & granulocytes. Lymph nodes and tonsils.

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  95 kDa & 170 kDa

• Additionnal Information:

  Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL

• References & Citations:

  Note: References for this clone sold by other suppliers may be listed for expected applications. J Exp Med (1990) 171, 1753-1771. (IP) J Applied Physiol (2003) 95(3): 969-976. (functional studies) J Leukocyte Biol (2008) 83: 728 -741. (IF) Meth Enzymol (2014) 537: 297-314. (Flow)

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4