Anti-Calprotectin (MRP14)(S100A9)(CAGB/426)

• Description:

  Calprotectin is a heteroduplex of Protein S100-A8/A9 (also called MRP-8/14, or Calgranulin A/B). This antibody recognizes the 14 kDa B component (Protein S100-A9) of the complex. Protein S100-A9 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. Altered expression of this protein is associated with the disease cystic fibrosis. This antibody reacts with neutrophils, monocytes, macrophages, and squamous mucosal epithelia and has been shown as an important marker for identifying macrophages in tissue sections.This antibody is available purified with BSA/azide at 200 ug/mL, or BSA/azide-free at 1 mg/mL.

• Synonyms:

  Calprotectin; Protein S100-A9; Myeloid-related protein 14; MRP-14; Calgranulin B; CAGB; Calprotectin L1H Subunit

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  S100A9

• Gene ID:

  6280 (S100A9 / Calgranulin B / MRP-14)

• NCBI Gene ID:

  112405 (S100A9 / Calgranulin B / MRP-14)

• UniProt:

  P06702 (S100A9 / Calgranulin B / MRP-14)

• Cellular Locus:

  Cytoplasmic|Cytoskeleton

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Recombinant human CAGB protein

• Target Antigen:

  Calprotectin | S100-A9

• Clonality:

  Monoclonal

• Isotype:

  IgM κ

• Clone:

  CAGB/426

• Conjugation:

  Purified, with BSA

• Source:

  Animal

• Applications:

  IHC, FFPE (verified)

• Validated Applications:

  IHC, FFPE

• Field of Research:

  Immunology, Inflammation

• Positive Control:

  Tonsil, lymph node, or spleen

• Concentration:

  0.2 mg/mL

• Buffer:

  PBS, 0.05% BSA, 0.05% azide

• Molecular Weight:

  14 kDa

• Additionnal Information:

  Immunohistology formalin-fixed. Not suitable for frozen tissues. 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Immunofluorescence 0.5-1 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4