Β-Glucosidase (β-GC) Activity Assay Kit

• Target:

  Β-GC

• Type:

  Colorimetric method

• Field of Research:

  Glycolysis and Lipid Metabolism; Plant Physiology Series

• Assay Principle:

  Β-glucosidase (β-GC), also known as β-glucoside hydrolase, can efficiently hydrolyze the terminal non-reducing β-D-glucoside bonds in various glycoside compounds, thereby releasing β-D-glucose and corresponding ligands. The enzyme is widely present in nature, including the seeds of various plants, the small intestine and other organs of animals, and microorganisms. β-GC is a very important industrial enzyme with various applications. In the field of medicine, β-GC catalyzes the degradation of glycoside groups to inhibit the expansion of cancer cells. In terms of diet, β-GC catalyzes the production of anti-cancer molecules isoflavones in soybean. In the energy field, β-GC is one of the key enzymes in the conversion of cellulose to glucose. β-GC catalyzed the reaction of substrate to produce chromogenic substances at the wavelength of 400 nm, the maximum absorption of β-GC enzyme activity was reflected by measuring the OD value at 400 nm.

• Assay Performance Time:

  50 min

• Sample Type:

  Tissue; fungus

• Detection Range:

  0.06-6.79 U/L

• Sensitivity:

  0.06 U/L

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  This product can be stored at -20°C for 12 months with shading light.

• Inter CV:

  2.0-9.7%

• Intra CV:

  2.8-4.5%

• Detection Instrument:

  Microplate reader