SET Recombinant Rabbit Monoclonal Antibody [JB60-34]

• Description:

  Multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone chaperoning. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T-lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Isoform 1 and isoform 2 inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins; however, isoform 2 specific activity is higher.

• Product Name Alternative:

  2PP2A antibody HLA DR associated protein II antibody HLA-DR-associated protein II antibody I 2PP2A antibody I-2PP2A antibody I2PP2A antibody IGAAD antibody Inhibitor of granzyme A activated DNase antibody Inhibitor of granzyme A-activated DNase antibody Inhibitor of GzmA-activated DNase antibody inhibitor-2 of protein phosphatase-2A antibody IPP2A2 antibody PHAPII antibody Phosphatase 2A inhibitor I2PP2A antibody protein phosphatase type 2A inhibitor antibody Protein SET antibody Set antibody SET nuclear oncogene antibody SET translocation antibody SET translocation (myeloid leukemia-associated) antibody SET_HUMAN antibody TAF I antibody TAF IBETA antibody TAF-I antibody TAFI antibody Template activating factor I antibody Template-activating factor I antibody Template-Activating Factor-I, chromatin remodelling factor antibody

• Abbreviation:

  IGAAD, I-2PP2A, TAF-I, SET

• Swiss Prot:

  Q01105 Human, Q9EQU5 Mouse

• Cellular Locus:

  Nucleus. Cytoplasm. Endoplasmic reticulum.

• Host:

  Rabbit

• Species Reactivity:

  Human, Mouse

• Immunogen:

  Recombinant protein within Human SET aa 160-290 / 290.

• Isotype:

  IgG

• Conjugation:

  Non-conjugated

• Type:

  Recombinant Rabbit monoclonal Antibody

• Applications:

  WB, IF-Cell, IHC-P, IP, IF-Tissue

• Positive Control:

  Rat kidney tissue, human tonsil tissue, mouse fallopian tube tissue, human lung carcinoma tissue, human kidney tissue, K-562 cell lysate, HeLa cell lysate, NIH/3T3 cell lysate.

• Concentration:

  1 mg/mL

• Dilution:

  WB: 1:500-1:2,000 ;IF-Cell: 1:50-1:200 ;IHC-P: 1:50-1:200 ;IP: 1-2μg/sample ;IF-Tissue: 1:50

• Purity:

  Protein A affinity purified.

• Form:

  Liquid

• Buffer:

  1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

• Molecular Weight:

  Predicted band size: 33 kDa

• Storage Conditions:

  Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

• Recombinant Antibody:

  Yes

• CAS Number:

  9000-83-3