Biotinylated Cynomolgus B7-H7 / HHLA2 Protein, His, Avitag™

• Background:

  B7-H7 (HHLA2) is a newly identified B7 family member that regulates human T-cell functions. B7-H7 was previously known as human endogenous retrovirus-H long terminal repeat associating 2 (HHLA2) with unidentified function. Recently, B7-H7 has been identified as a specific ligand for human CD28H. The B7-H7-CD28H pathway strongly promoted CD4+ T-cell proliferation and cytokine production via an AKT-dependent signaling cascade in the presence of TCR signaling, suggesting B7-H7 comprises a new co-stimulatory pathway. The first IgV domain of B7-H7, which presumably binds to a putative receptor, shows the highest homology to other B7 family members.

• Description:

  Biotinylated Cynomolgus B7-H7, His, Avitag (B77-C82E9) is expressed from human 293 cells (HEK293) . It contains AA Ile 21 - Asn 345 (Accession # XP_005548285.1) .

• Specifications:

  This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 39.1 kDa. The protein migrates as 66-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

• Accession Number:

  XP_005548285.1

• Expression Region:

  Ile 21 - Asn 345

• Host:

  HEK293

• Target:

  B7-H7

• Conjugation:

  Biotin-labeled

• Tag:

  C-10xHis & C-Avi

• Source:

  Cynomolgus

• Stability:

  ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

• Endotoxin:

  1.0 EU per μg

• Purity:

  90%

• Bioactivity:

  Immobilized Biotinylated Cynomolgus B7-H7, His,Avitag (Cat. No. B77-C82E9) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human CD28H, Fc Tag (Cat. No. CDH-H5251) with a linear range of 0.1-8 ng/mL (QC tested).

• Format:

  Powder

• Buffer:

  PBS, pH7.4

• Additives:

  Trehalose

• Molecular Weight:

  39.1 kDa

• Shipping Conditions:

  RT

• Storage Conditions:

  -20°C

• Package Size:

  25ug*1

• Species:

  Cynomolgus