Biotinylated SARS-CoV-2 Nucleocapsid protein, His, Avitag™ (BA.2*&BA.3*/Omicron)

• Background:

  Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology; 517: 62-68) . The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.

• Description:

  Biotinylated SARS-CoV-2 Nucleocapsid protein, His, Avitag (BA.2*&BA.3*/Omicron) (NUN-C82E9) is expressed from human 293 cells (HEK293) . It contains AA Met 1 - Ala 419 (Accession # QHO62115.1 (P13L, ERS31-33del, R203K, G204R, S413R) ) . The nucleocapsid mutations are identified on the SARS-CoV-2 Omicron variant (Pango lineage: BA.2; GISAID clade: GRA; Nextstrain clade: 21L & Pango lineage: BA.3; GISAID clade: GRA; Nextstrain clade: 21M) .

• Specifications:

  This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 48.8 kDa. The protein migrates as 33 kDa and 50-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

• Accession Number:

  QHO62115.1 (P13L, ERS31-33del, R203K, G204R, S413R)

• Expression Region:

  Met 1 - Ala 419

• Host:

  HEK293

• Target:

  Nucleocapsid protein

• Conjugation:

  Biotin-labeled

• Tag:

  C-6xHis & C-Avi

• Source:

  SARS-CoV-2

• Stability:

  ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

• Endotoxin:

  1.0 EU per μg

• Purity:

  90%

• Bioactivity:

  Immobilized Anti-SARS-CoV-2 Nucleocapsid Antibody, Chimeric mAb, Human IgG1 (Cat. No. NUN-CH15) at 1 μg/mL (100 μL/well) can bind Biotinylated SARS-CoV-2 Nucleocapsid protein, His,Avitag (BA.2*&BA.3*/Omicron) (Cat. No. NUN-C82E9) with a linear range of 0.1-6 ng/mL (QC tested).

• Format:

  Powder

• Buffer:

  PBS, 0.2 M Arginine, pH7.4

• Additives:

  Trehalose

• Molecular Weight:

  48.8 kDa

• Shipping Conditions:

  RT

• Storage Conditions:

  -20°C

• Package Size:

  25ug*1

• Species:

  SARS-CoV-2