Cynomolgus Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer Protein, His Tag&Tag Free (MALS verified)

• Background:

  Integrin alpha V beta 8 (ITGAV & ITGB8 or ITGAVB8) is expressed in yolk sac, placenta, brain perivascular astrocytes, Schwann cells, renal glomerular mesangial cells and pulmonary epithelial cells. Unlike other alpha V integrins, ITGAVB8 does not appear to assume different activation states, and the cytoplasmic tail does not connect to the cytoskeleton. It binds ligands containing an RGD motif, including vitronectin, fibrin and the latency associated peptide (LAP) of the latent TGF-beta complex. High affinity binding of alpha V beta 8 to LAP allows proteolytic cleavage by MT1-MMP, which releases active TGF-beta. This mechanism differs from that of alpha V beta 6, the other alpha V integrin which can activate TGF-beta from latency through non-proteolytic mechanisms. Downstream effects of TGF-beta activation include control of cell growth and associated vascularization.

• Description:

  Cynomolgus ITGAV&ITGB8 Heterodimer Protein, His Tag&Tag Free (IT8-C52W9) is expressed from human 293 cells (HEK293) . It contains AA Phe 31 - Pro 993 (ITGAV) & Glu 43 - Ser 681 (ITGB8) (Accession # A0A2K5WCD3-1 (ITGAV) & G7P0S0-1 (ITGB8) ) .

• Specifications:

  Cynomolgus Integrin alpha V beta 8 (ITGAV&ITGB6) Heterodimer Protein, produced by co-expression of ITGAV and ITGB8, has a calculated MW of 113.1 kDa (ITGAV) and 76.0 kDa (ITGB8) . Subunit ITGAV is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB8 contains no tag but a basic tail at the C-terminus. The predicted N-terminus is Phe 31 (ITGAV) & Glu 43 (ITGB8) . The non-reducing (NR) protein migrates as 135-150 kDa (ITGAV) & 75-85 kDa (ITGB8) respectively due to glycosylation.

• Accession Number:

  A0A2K5WCD3-1 (ITGAV) & G7P0S0-1 (ITGB8)

• Expression Region:

  Phe 31 - Pro 993 (ITGAV) & Glu 43 - Ser 681 (ITGB8)

• Host:

  HEK293

• Target:

  Integrin alpha V beta 8

• Conjugation:

  Unconjugated

• Tag:

  C-10xHis | Native

• Source:

  Cynomolgus

• Stability:

  ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

• Endotoxin:

  1.0 EU per μg

• Purity:

  95%

• Bioactivity:

  Immobilized Cynomolgus ITGAV&ITGB8 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT8-C52W9) at 5 μg/mL (100 μL/well) can bind Biotinylated Human Latent TGF-Beta 1, His,Avitag (Cat. No. TG1-H82Qb) with a linear range of 0.5-16 ng/mL (QC tested).

• Format:

  Powder

• Buffer:

  50 mM Tris, 150 mM NaCl, pH7.5

• Additives:

  Trehalose

• Molecular Weight:

  113.1 kDa (ITGAV) & 76.0 kDa (ITGB8)

• Shipping Conditions:

  RT

• Storage Conditions:

  -20°C

• Package Size:

  100ug*1

• Species:

  Cynomolgus