Anti-Hu CD166 APC

• Description:

  CD166 is a type 1 transmembrane glycoprotein, which is anchored to the actin cytoskeleton through its intracellular domain, whereas its extracellular part binds its ligand CD6. CD166 functions as an adhesion molecule expressed during development of many organs, as well as in hematopoiesis (pluripotent stem cells), endothelial and epithelial linings, endometrium, blastocysts, and in the central and peripheral nervous system (on neurons), as well as on activated T cells and activated monocytes. Multiple isoforms of CD166 have been found.

• Certification:

  RUO

• Immunogen:

  Cultured human thymic epithelial cells

• Target Antigen:

  CD166

• Clone:

  3A6

• Applications:

  FC (QC tested)

• Format:

  APC

• Buffer:

  Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• References & Citations:

  *Leukocyte Typing VI., Kishimoto T et al. (editors), Garland Publishing Inc. (1997) ., URL: , *Kijima N, Hosen N, Kagawa N, Hashimoto N, Nakano A, Fujimoto Y, Kinoshita M, Sugiyama H, Yoshimine T: CD166/activated leukocyte cell adhesion molecule is expressed on glioblastoma progenitor cells and involved in the regulation of tumor cell invasion. Neuro Oncol. 2012 Oct;14 (10) :1254-64., URL: https://www.ncbi.nlm.nih.gov/pubmed/22166264, *Piazza T, Cha E, Bongarzone I, Canevari S, Bolognesi A, Polito L, Bargellesi A, Sassi F, Ferrini S, Fabbi M: Internalization and recycling of ALCAM/CD166 detected by a fully human single-chain recombinant antibody. J Cell Sci. 2005 Apr 1;118 (Pt 7) :1515-25., URL: https://pubmed.ncbi.nlm.nih.gov/15769845/, *Levesque MC, Heinly CS, Whichard LP, Patel DD: Cytokine-regulated expression of activated leukocyte cell adhesion molecule (CD166) on monocyte-lineage cells and in rheumatoid arthritis synovium. Arthritis Rheum. 1998 Dec;41 (12) :2221-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/9870879, *Pretzel D, Linss S, Rochler S, Endres M, Kaps C, Alsalameh S, Kinne RW: Relative percentage and zonal distribution of mesenchymal progenitor cells in human osteoarthritic and normal cartilage. Arthritis Res Ther. 2011 Apr 15;13 (2) :R64, URL: https://www.ncbi.nlm.nih.gov/pubmed/21496249, *Virtanen S, Schulte R, Stingl J, Caldas C, Shehata M: High-throughput surface marker screen on primary human breast tissues reveals further cellular heterogeneity. Breast Cancer Res. 2021 Jun 13;23 (1) :66., URL: https://pubmed.ncbi.nlm.nih.gov/34120626/

• Storage Conditions:

  Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

• Specificity:

  The mouse monoclonal antibody 3A6 recognizes an extracellular epitope of CD166, a type I transmembrane glycoprotein expressed on neurons, activated T cells, activated monocytes, endothelium, epithelium, endometrium, fibroblasts, pluripotent stem cells, and blastocysts.

• Applications Notes:

  6

• Species:

  Human

• CAS Number:

  9007-83-4

• Isotype:

  Mouse IgG1 kappa