Mouse anti-HER2 Antibody

• Background:

  Mouse monoclonal antibody to HER2

• Synonyms:

  HER2; MLN19; NEU; NGL; Receptor tyrosine-protein kinase erbB-2; Metastatic lymph node gene 19 protein; MLN 19; Proto-oncogene Neu; Proto-oncogene c-ErbB-2; Tyrosine kinase-type cell surface receptor HER2; p185erbB2; CD340

• Host:

  Mouse

• Reactivity:

  H

• Immunogen:

  KLH-conjugated synthetic peptide encompassing a sequence of human HER2. The exact sequence is proprietary.

• Clonality:

  Monoclonal

• Applications:

  WB, IHC

• Concentration:

  1 mg/mL

• Dilution:

  WB (1/1000 - 1/2000), IH (1/200 - 1/500)

• Buffer:

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

• Storage Conditions:

  Store at -20°C. Avoid repeated freeze / thaw cycles.

• WB Description:

  Western blot analysis of HER2 expression in Hela (A), 293 (B), Jurkat (C) whole cell lysates.

• IHC description:

  Immunohistochemical analysis of HER2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• Gene ID (Human):

  2064

• SwissProt (Human):

  P04626

• CAS Number:

  9007-83-4