Rabbit anti-SFRP4 Antibody

• Background:

  Rabbit polyclonal antibody to SFRP4

• Synonyms:

  FRPHE; Secreted frizzled-related protein 4; sFRP-4; Frizzled protein, human endometrium; FrpHE

• Host:

  Rabbit

• Reactivity:

  H, M, R

• Immunogen:

  Recombinant full length protein of human SFRP4

• Clonality:

  Polyclonal

• Applications:

  WB, IHC, IF/IC

• Purification Method:

  The antibody was purified by immunogen affinity chromatography.

• Concentration:

  1 mg/mL

• Dilution:

  WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/100)

• Buffer:

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

• Storage Conditions:

  Store at -20°C. Avoid repeated freeze / thaw cycles.

• WB Description:

  Western blot analysis of SFRP4 expression in HepG2 (A), Hela (B) whole cell lysates.

• IHC description:

  Immunohistochemical analysis of SFRP4 staining in human ovarian cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• IF/ICC description:

  Immunofluorescent analysis of SFRP4 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

• Gene ID (Human):

  6424

• Gene ID (Mouse):

  20379

• Gene ID (Rat):

  89803

• SwissProt (Human):

  Q6FHJ7

• SwissProt (Mouse):

  Q9Z1N6

• SwissProt (Rat):

  Q9JLS4

• CAS Number:

  9007-83-4