Rabbit anti-NF-kappaB p65 Antibody

• Background:

  Rabbit polyclonal antibody to NF-kappaB p65

• Synonyms:

  NFKB3; Transcription factor p65; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3

• Host:

  Rabbit

• Reactivity:

  H, M, R, B, Mk, P

• Immunogen:

  KLH-conjugated synthetic peptide encompassing a sequence within the center region of human NF-kappaB p65. The exact sequence is proprietary.

• Clonality:

  Polyclonal

• Applications:

  WB, IHC

• Purification Method:

  The antibody was purified by immunogen affinity chromatography.

• Concentration:

  1 mg/mL

• Dilution:

  WB (1/500 - 1/1000), IH (1/100 - 1/200)

• Buffer:

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

• Storage Conditions:

  Store at -20°C. Avoid repeated freeze / thaw cycles.

• WB Description:

  Western blot analysis of NF-kappaB p65 expression in HeLa (A), Raw264.7 (B), mouse liver (C), rat kidney (D) whole cell lysates.

• IHC description:

  Immunohistochemical analysis of NF-kappaB p65 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• Gene ID (Human):

  5970

• Gene ID (Mouse):

  19697

• SwissProt (Human):

  Q04206

• SwissProt (Mouse):

  Q04207

• CAS Number:

  9007-83-4