Rabbit anti-Beta-tubulin Antibody

• Background:

  Rabbit polyclonal antibody to Beta-tubulin

• Synonyms:

  TUBB4; Tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-III

• Host:

  Rabbit

• Reactivity:

  H, M, R

• Immunogen:

  Recombinant protein corresponding to human Beta-tubulin.

• Clonality:

  Polyclonal

• Applications:

  WB, IHC

• Purification Method:

  The antibody was purified by immunogen affinity chromatography.

• Concentration:

  1 mg/mL

• Dilution:

  WB (1/1000 - 1/3000), IH (1/50 - 1/100)

• Buffer:

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,0.2% BSA, 30% glycerol, and 0.01% sodium azide.

• Storage Conditions:

  Store at -20°C. Avoid repeated freeze / thaw cycles.

• WB Description:

  Western blot analysis of Beta-tubulin expression in Hela (A), mouse brain (B), rat brain (C) whole cell lysates.

• IHC description:

  Immunohistochemical analysis of Beta-tubulin staining in mouse heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• Gene ID (Human):

  10381

• Gene ID (Mouse):

  22152

• Gene ID (Rat):

  246118

• SwissProt (Human):

  Q13509

• SwissProt (Mouse):

  Q9ERD7

• SwissProt (Rat):

  Q4QRB4

• CAS Number:

  9007-83-4