AKT1 (Prognostic Marker for Neuroendocrine Tumors) (AKT1/2784), CF405S conjugate, 0.1mg/mL

This antibody recognizes a protein of 62 kDa, which is identified as AKT1. The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 (also designated PKBbeta or RacPK-beta) and Akt3 (also designated PKB-gamma or thymoma viral proto-oncogene 3), which exhibit sequence homology with the protein kinase A and C families and are encoded by the c-Akt proto-oncogene. All members of the Akt family have a Pleckstrin homology domain. Akt1 and Akt2 are activated by PDGF stimulation. This activation is dependent on PDGFR-beta tyrosine residues 740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1 (IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Akt proteins become phosphorylated and activated in insulin/IGF-1-stimulated cells by an upstream kinase (s), and the activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

9007-83-4

AKT1; PKB; Protein Kinase B Alpha; RAC-alpha serine/threonine-protein kinase

41116161

Primary and secondary antibodies for multiple methodology immunostaining detection application

AKT1

207

525622

P31749

P31749

AKT1

Recombinant fragment of human AKT1 protein (around aa 85-189) (exact sequence is proprietary)

AKT1

AKT1/2784

Primary

Animal

Animal

IHC, FFPE (verified) |WB (verified)

PDGF-treated NIH/3T3 cells. HeLa cell lysates. Human pancreas or cervical carcinoma.

2 years

0.1 mg/mL

PBS, 0.1% BSA, 0.05% azide

62 kDa

RT (ND)

Stable at room temperature or 37°C (98°F) for 7 days.

Protect from light

Guaranteed for at least 24 months from date of receipt when stored as recommended

IgG

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

0.05% sodium azide (CAS 26628-22-8)