Anti-ATG5 (Autophagy Marker)(ATG5/2553)

• Description:

  Autophagy protein 5 (ATG5), in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. ATG5 is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. The ATG5 protein is essential for autophagy; a process that is usually beneficial for cells to self-degrade their own components when they are no longer useful. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  Autophagy protein 5; ATG5; Autophagy related 5; APG5-like; Apoptosis-specific protein

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  ATG5

• Gene ID:

  9474

• NCBI Gene ID:

  486063

• UniProt:

  Q9H1Y0

• Cellular Locus:

  Cytoplasmic

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Recombinant fragment of human ATG5 protein (around aa 1-119) (exact sequence is proprietary)

• Target Antigen:

  ATG5 | Autophagy protein 5

• Clonality:

  Monoclonal

• Isotype:

  IgG1 κ

• Clone:

  ATG5/2553

• Conjugation:

  Purified, BSA-free

• Source:

  Animal

• Applications:

  IHC, FFPE (verified) | WB (verified)

• Validated Applications:

  IHC, FFPE, WB

• Positive Control:

  K562, U-87, A431, THP-1, PANC-1, Raji or HeLa cells. Ovary, Endometrium, Colon or Duodenum.

• Concentration:

  1 mg/mL

• Buffer:

  PBS, no BSA, no azide

• Molecular Weight:

  32 kDa

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  -35°C to -5°C ; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4