Anti-CD25 / IL2RA (Activated Lymphocyte Marker)(IL2RA/2394), Biotin conjugate

• Description:

  Recognizes a protein of 55 kDa, identified as CD25. It is expressed on activated T- and B-cells and activated monocytes/macrophages. With respect to lymphomas, CD25 is present on malignant cells of Hodgkin's disease, HTLV-1 adult T-cell leukemia, cutaneous T-cell lymphoma, and hair cell leukemia. Increased levels of soluble CD25 are observed in the leukemias/lymphomas and inflammatory/ autoimmune diseases. CD25 molecule alone appears to function as a low affinity receptor and associates with CD122 (IL-2R chain, p75) and CD132 (common chain) to form the high affinity IL-2 receptor complex. CD25 antibodies detect three epitope regions, A, B and C. This MAb recognizes the epitope B, which is located at residue 3-104 of CD25 and doe not block IL-2 binding to CD25. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  IL2 Receptor alpha; IL2R alpha chain; IL2R; IL2R subunit alpha IL2RA; Interleukin-2 receptor subunit alpha; Ly43; p55; p55 chain; T Cell Growth Factor Receptor; TAC antigen; TCGFR

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  IL2RA

• Gene ID:

  3559

• NCBI Gene ID:

  231367

• UniProt:

  P01589

• Cellular Locus:

  Plasma membrane

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Recombinant human IL2RA protein fragment (around aa 42-183) (exact sequence is proprietary)

• Target Antigen:

  CD25 | IL2RA

• Clonality:

  Monoclonal

• Isotype:

  IgG2b κ

• Clone:

  IL2RA/2394

• Conjugation:

  Biotin

• Disease:

  Tumor

• Source:

  Animal

• Positive Control:

  PHA-stimulated human lymphocytes. Human lymph node or tonsil.

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  55 kDa

• Additionnal Information:

  For coating for ELISA, order Ab without BSA|Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Optimal dilution and staining procedure for a specific application should be determined by user|Recommended starting concentrations for titration are 1-2 ug/mL for most applications, or 1 ug/million cells/100 uL for flow cytometry

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4