Anti-CLEC9A(1F6)

• Description:

  This Mab recognizes Mouse DNGR-1, which is also known as CLEC9A, which is a highly specific marker of the CD8a and the CD103 DC subsets, as well as a receptor able to recognize a preformed signal exposed on necrotic cells. In peripheral blood, it is highly restricted on the surface of BDCA31( ) dendritic cells and on a small subset of CD14( ) and CD16(-) monocytes._x000D_ _x000D_ Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_ _x000D_

• Synonyms:

  C type lectin domain family 9; member A; C-type lectin domain family 8 member A; CLEC9A; DNGR1; HEEE9341; PRO34046; UNQ9341; CD370

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene ID:

  232414

• NCBI Gene ID:

  391518

• UniProt:

  Q8RU4

• Cellular Locus:

  Plasma membrane

• Host:

  Rat

• Species Reactivity:

  Mouse

• Immunogen:

  RBL-2H3 cells expressing Mouse CLEC9A fused to an HA epitope.

• Target Antigen:

  CLEC9A

• Clonality:

  Monoclonal

• Isotype:

  IgG1 κ

• Clone:

  1F6

• Conjugation:

  Purified, with BSA

• Source:

  Animal

• Applications:

  Flow, surface (published) | Functional studies (published)

• Validated Applications:

  FC, Functional assays

• Positive Control:

  Mouse spleen.

• Concentration:

  0.2 mg/mL

• Buffer:

  PBS, 0.05% BSA, 0.05% azide

• Molecular Weight:

  27 kDa

• Additionnal Information:

  Flow cytometry: 0.5-1 ug/million cells; Western blot 0.5-1 ug/mL; Immunofluorescence: 0.5-1 ug/mL; Optimal dilution for a specific application should be determined.|Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody

• References & Citations:

  Note: References for this clone sold by other suppliers may be listed for expected applications. Nature (2009) 458(7240): 899-903. (functional studies) J Immunol (2015) 195:5066-5076. (Flow, surface)

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4