HIF1 alpha (Hypoxia-Inducible Factor 1-alpha) (ESEE122), CF640R conjugate, 0.1mg/mL

HIF1 (hypoxia-inducible factor 1), a heterodimeric transcription factor complex central to cellular response to hypoxia, consists of two subunits (HIF-1 alpha and HIF-1 beta) which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family. Expression of HIF-1 alpha protein is regulated by cellular oxygen level alterations as well as in oxygen-independent manner via different cytokines (through the PI3K-AKT-mTOR pathway), growth factors, oncogenic activation, or loss of tumor suppressor function etc. In normoxic cells, HIF-1 alpha is proline hydroxylated leading to a conformational change that promotes its binding to the VLH (von Hippel Lindau) protein E3 ligase complex; ubiquitination and followed by rapid proteasomal degradation. Hypoxia as well as chemical hydroxylase inhibitors (desferrioxamine, cobalt etc.) inhibit HIF-1 alpha degradation and lead to its accumulation in the cells, whereas, contrastingly, HIF-1 beta/ARNT (AhR nuclear translocator) remains stable under both conditions. Besides their critical role in hypoxic response, HIF1s regulates the transcription of genes responsible for angiogenesis, erythropoiesis/iron-metabolism, glucose metabolism, cell proliferation/survival, adipogenesis, carotid body formation, B lymphocyte development and immune reactions. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

9007-83-4

ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78 (bHLHe78) ; Member of PAS superfamily 1 (MOP1) ; PAS domain-containing protein 8 (PASD8)

41116161

Primary and secondary antibodies for multiple methodology immunostaining detection application

HIF1A

3091

597216

Q16665

Q16665

HIF1 Alpha

GST-human HIF-1A amino acids 329-530 fusion protein

HIF1 Alpha

ESEE122

Primary

Animal

Animal

IHC, FFPE (published) |WB (published)

Cobalt chloride treated HeLa cells. Colon, Renal, Breast or Bladder Carcinomas.

2 years

0.1 mg/mL

PBS, 0.1% BSA, 0.05% azide

92-110 kDa

Note: References for this clone sold by other suppliers may be listed for expected applications. Cancer (2006) 107(4): 757-766. (IHC, FFPE) Biol Pharm Bull (2017) 40(1): 82-87. (WB)

RT (ND)

Stable at room temperature or 37°C (98°F) for 7 days.

Protect from light

Guaranteed for at least 24 months from date of receipt when stored as recommended

IgG1, kappa

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

0.05% sodium azide (CAS 26628-22-8)