Biotinylated Human B7-H7 / HHLA2 Protein, Fc, Avitag™

• Background:

  B7-H7 (HHLA2) is a newly identified B7 family member that regulates human T-cell functions. B7-H7 was previously known as human endogenous retrovirus-H long terminal repeat associating 2 (HHLA2) with unidentified function. Recently, B7-H7 has been identified as a specific ligand for human CD28H. The B7-H7-CD28H pathway strongly promoted CD4+ T-cell proliferation and cytokine production via an AKT-dependent signaling cascade in the presence of TCR signaling, suggesting B7-H7 comprises a new co-stimulatory pathway. The first IgV domain of B7-H7, which presumably binds to a putative receptor, shows the highest homology to other B7 family members.

• Description:

  Biotinylated Human B7-H7, Fc, Avitag (B77-H82F5) is expressed from human 293 cells (HEK293) . It contains AA Ile 23 - Asn 344 (Accession # Q9UM44-1) .

• Specifications:

  This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 65.9 kDa. The protein migrates as 80-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

• Accession Number:

  Q9UM44-1

• Expression Region:

  Ile 23 - Asn 344

• Host:

  HEK293

• Target:

  B7-H7

• Conjugation:

  Biotin-labeled

• Tag:

  C-Fc & C-Avi

• Source:

  Human

• Stability:

  ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

• Endotoxin:

  1.0 EU per μg

• Purity:

  90%

• Bioactivity:

  Immobilized Human CD28H, Fc Tag (Cat. No. CDH-H5251) at 5 μg/mL (100 μL/well) can bind Biotinylated Human B7-H7, Fc,Avitag (Cat. No. B77-H82F5) with a linear range of 5-78 ng/mL (QC tested).

• Format:

  Powder

• Buffer:

  Tris with Glycine, Arginine and NaCl, pH7.5

• Additives:

  Trehalose

• Molecular Weight:

  65.9 kDa

• Shipping Conditions:

  RT

• Storage Conditions:

  -20°C

• Package Size:

  200ug*1

• Species:

  Human