Noggin Recombinant

• Background:

  Noggin is one of a group of proteins that act as secreted antagonists of BMP activity. BMPs are essential for osteogenesis and organogenesis during embryonic development, and also play a role in tissue healing in adults. Noggin inhibits BMP activity by binding to them with high affinity, blocking their ability to bind to their receptor.

• Description:

  Human Noggin, also known as symphalangism 1 (SYM1) or synostoses (multiple) syndrome 1 (SYNS1), GenBank Accession No. NM_005450, a.a. 28-232 (end), disulfide-linked homodimer. MW=~28kDa (monomer) . Source: human 293 cells. Endotoxin Level: <1 EU/μg. Biological Activity: measured by Noggin’s ability to inhibit BMP-4-induced alkaline phosphatase production by C2C12 mouse myoblast cells. The ED50 is < 20 ng/ml in the presence of 30 ng/ml of human BMP-4. Formulation: Lyophilized from a 0.2 μM filtered, aqueous solution. Reconstitution: Supplied as a lyophilized solid. Reconstitute in sterile water with 0.1% BSA to a final concentration of 0.1 mg/ml. Final formulation will be 50 mM Tris, pH 8.0,1.2 M NaCl, 2.7 mM KCl, 0.1% BSA.

• UniProt:

  Q13253

• Tag:

  None

• Applications:

  Useful for cell culture and for the study of signaling and developmental pathways. Also useful for receptor binding studies, screening inhibitors, and selectivity profiling.

• Endotoxin:

  < 1 EU per µg of Noggin as determined by the LAL method.

• Format:

  Lyophilized solid

• Reconstitution:

  Reconstitute in sterile water with 0.1% BSA to a final concentration of 0.1 mg/ml. Final formulation will be 50 mM Tris, pH 8.0,1.2 M NaCl, 2.7 mM KCl, 0.1% BSA. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.

• Shipping Conditions:

  -80°C

• Storage Conditions:

  ≥12 months (lyophilized) at -20°C. Store reconstituted protein in aliquots at -20°C to –70°C for up to 6 months. Avoid freeze/thaw cycles.

• Calculated Molecular Weight:

  28 kDa

• Formulation:

  Lyophilized from a 0.2 µm filtered 0.1 mg/ml solution of 50 mM Tris, pH 8.0,1.2 M NaCl, 2.7 mM KCl, 0.1% BSA