CD47:SIRP-α[Biotinylated] Inhibitor Screening Assay Kit

• Background:

  Signal-regulatory protein alpha (SIRP-α), also known as CD172a, is a cell surface protein expressed mainly by myeloid cells such as macrophages. Its receptor, CD47, is ubiquitously expressed on the surface of all cells and has been found to be overexpressed in some cancers. CD47 is a macrophage immune checkpoint, and binding to SIRP-α delivers a “Do Not Eat Me” signal to macrophages, leading to inhibition of phagocytosis of tumor cells. In the context of a tumor, this signaling aids in tumor evasion of the immune system. The levels of CD47 mRNA have been correlated to survival rate in solid tumors. Inhibitors of this interaction hold great promise for cancer immunotherapy. For instance, anti-CD47 antibodies have been shown to induce a phenotype switch from M2 to M1 TAM (tumor infiltrating macrophages) and target tumor cells. The use of anti-CD47 in combination with other therapies, such as chemotherapy, seem also to result in better outcomes.

• Description:

  The CD47:SIRP-α[Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit is an ELISA-based assay designed to measure the binding between CD47 and SIRP-α (Signal-regulatory protein alpha) protein for screening and profiling applications. This kit comes with enough purified CD47 (amino acids 19-139) and biotinylated SIRP-α (amino acids 27-370), streptavidin-HRP, assay buffer, and detection reagent for 100 enzyme reactions.Figure 1. CD47:SIRP-α[Biotinylated] Inhibitor Screening ChemiluminescenceAssay Kit schematic.A 96-well plate is coated with CD47 protein. After coating and blocking, biotinylated SIRP-α is added in an optimized assay buffer. Next, unbound biotinylated SIRP-α is washed away, and the plate is incubated with streptavidin-HRP. After a final wash, ELISA ECL substrate is added to produce chemiluminescence that can be measured using a chemiluminescence reader. The chemiluminescence signal is proportional to the efficacy of SIRP-α binding to CD47.Need us to run inhibitor screens or profile your compounds against CD47:SIRP-α[Biotinylated]? Check out ourImmunotherapy Biochemical Screening Services.

• UniProt:

  CD47: Q08772; SIRP-α: P78324

• Applications:

  Study and screen compounds that inhibit the binding of CD47 to SIRP-α for drug discovery in high throughput screening (HTS) applications.

• Format:

  Catalog #NameAmountStorage71177CD47, Fc fusion (IgG1) *10 µg-80°C71138SIRP-α (CD172a), His-tag, Biotin-labeled*60 µg-80°C79742Streptavidin-HRP10 µl+4°C793113x Immuno Buffer 150 ml-20°C79728Blocking Buffer 250 ml+4°C79670ELISA ECL substrate A (transparent bottle) 6 mlRoom TempELISA ECL substrate B (brown bottle) 6 mlRoom Temp79699White 96-well microplate1Room Temp*The concentration of the protein is lot-specific and will be indicated on the tube.

• Shipping Conditions:

  -80°C

• Storage Conditions:

  This assay kit will perform optimally for up to 6 monthsfrom date of receipt when the materials are storedas directed.

• Notes:

  Visitbpsbioscience.com/assay-kits-faqfor detailed troubleshooting instructions. For all further questions, please email[email protected]

• Contraindications:

  The CD47:SIRP-α[Biotinylated] Inhibitor Assay Kit is compatible with up to 1% final DMSO concentration.