Rabbit anti Human cyclin dependent kinase 2

• Background:

  Cyclin-Dependent Kinase 2 (CDK2), also known as p33cdk2 is expressed earlier in the cell cycle than is p34cdc2. Like p34cdc2, p33cdk2 associates with cyclin A in Human cells. However, kinase activity associated with cyclin A-cdc2 is found only in G2-phase. Cdk2 also complexes with cyclins E, D1, and D3. Cyclin E-cdk2 kinase is active in the G1- and S-phases of the cell cycle and is important (as does cyclin A-cdk2) for the progression from G1-to S-phase. The levels of cyclin A-cdk2 is maximal at the G1/S transition and both cdk2 and cyclin A are found associated with DNA in the initiation complex during replication. Rb protein acts as substrate for cdk2-cyclin E and/or cdk2-cyclin A in vivo. Cdk2 is activated and specifically localized to the nucleus during late G1-, S-, and G2-phase.

• Synonyms:

  Anti-Cyclin-Dependent Kinase-2, Phosphorylated, p33cdk2

• UniProt:

  P24941

• Host:

  Rabbit

• Species Reactivity:

  Human

• Isotype:

  IgG

• Conjugation:

  Unconjugated

• Type:

  Polyclonal Antibody

• Source:

  Rabbits were immunized with a synthetic peptide derived from the phosphorylated Human cdk2 protein.

• Applications:

  WB, IP

• Field of Research:

  Cell Cycle

• Purification Method:

  Ammonium Sulfate Precipitation

• Assay Principle:

  Antibody can be used for Western blotting (1:2,000 dilution) and immunoprecipitation. Optimal concentration should be evaluated by serial dilutions.

• Stability:

  See expiration date on vial

• Concentration:

  See vial for Concentration

• Form:

  This antibody recognizes only phosphorylated cdk2 protein.Provided as solution in phosphate buffered saline with 0,08% sodium azide

• Precautions:

  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.

• References & Citations:

  1. Coates, S., et al. 'A new pathway for mitogen-dependent cdk2 regulation uncovered in p27(Kip1)-deficient cells.' Curr. Biol. 1999, 9, 163-173_x000D_ 2. Lacy, S. , Whyte, P. 'Identification ofa p130 domain mediating interactions with cyclin A/cdk2 and cyclin E/cdk2 complexes.' Oncogene 1997, 14, 2395-2406

• Shipping Conditions:

  Ambient Temperature, freeze upon arrival

• Storage Conditions:

  Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles

• Functional Analysis:

  WB

• CAS Number:

  9007-83-4