Biotinylated Human TROP-2 / TACSTD2 Protein, Fc, Avitag™

• Background:

  TROP-2 is a single-copy gene in human cells, and encodes a type-1 transmembrane glycoprotein which is over-expressed in various malignancies, also referred to as tumor associated calcium signal transducer 2 (TACSTD2), GA733-1 or M1S1. TROP-2 is related to epithelial cell adhesion molecule (EpCAM), also called TROP-1, gp40, and KSA. Trop-1 and Trop-2 are homologous to serum IGF-II-binding proteins and appear as signal transducers. Thus, they likely represent novel cell-surface receptors and may play a role in regulating the growth of carcinoma cells.

• Description:

  Biotinylated Human TROP-2 Protein, Fc, Avitag (TR2-H82F4) is expressed from human 293 cells (HEK293) . It contains AA Gln 31 - Thr 274 (Accession # P09758-1) .

• Specifications:

  This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 55.6 kDa. The protein migrates as 66-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

• Accession Number:

  P09758-1

• Expression Region:

  Gln 31 - Thr 274

• Host:

  HEK293

• Target:

  TROP-2

• Conjugation:

  Biotin-labeled

• Tag:

  C-Fc & C-Avi

• Source:

  Human

• Stability:

  ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

• Endotoxin:

  1.0 EU per μg

• Purity:

  90%

• Bioactivity:

  Immobilized Mouse Monoclonal Antibody Against Human TROP-2 Protein,Mouse IgG1 at 1 μg/mL (100 μL/well) can bind Biotinylated Human TROP-2 Protein, Fc,Avitag (Cat. No. TR2-H82F4) with a linear range of 0.2-16 ng/mL (QC tested).

• Format:

  Powder

• Buffer:

  50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5

• Additives:

  Trehalose

• Molecular Weight:

  55.6 kDa

• Shipping Conditions:

  RT

• Storage Conditions:

  -20°C

• Package Size:

  200ug*1

• Species:

  Human