Anti-Hu CD105 Pacific Blue™

• Description:

  CD105 (endoglin) is a homodimeric transmembrane glycoprotein serving in presence of TGFbetaR-2 as a receptor for TGFbeta-1 and TGFbeta-3. CD105 is highly expressed on endothelial cells and promotes angiogenesis during wound healing, infarcts and in a wide range of tumours and its gene expression is stimulated by hypoxia. CD105 prevents apoptosis in hypoxic endothelial cells and also antagonises the inhibitory effects of TGFbeta-1 on vascular endothelial cell growth and migration. Normal cellular levels of CD105 are required for formation of new blood vessels.

• Certification:

  RUO

• Immunogen:

  Vaccinia

• Target Antigen:

  CD105

• Clone:

  MEM-226

• Applications:

  FC (QC tested)

• Format:

  Pacific Blue™

• Buffer:

  Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• References & Citations:

  *Schmidt D, Achermann J, Odermatt B, Breymann C, Mol A, Genoni M, Zund G, Hoerstrup SP: Prenatally fabricated autologous human living heart valves based on amniotic fluid derived progenitor cells as single cell source. Circulation. 2007 Sep 11;116 (11 Suppl) :I64-70., URL: http://www.ncbi.nlm.nih.gov/pubmed/17846327, *Asumda FZ, Chase PB: Age-related changes in rat bone-marrow mesenchymal stem cell plasticity. BMC Cell Biol. 2011 Oct 12;12:44., URL: https://www.ncbi.nlm.nih.gov/pubmed/21992089 , *Liang L, Zheng D, Lu C, Xi Q, Bao H, Li W, Gu Y, Mao Y, Xu B, Gu X: Exosomes derived from miR-301a-3p-overexpressing adipose-derived mesenchymal stem cells reverse hypoxia-induced erectile dysfunction in rat models. Stem Cell Res Ther. 2021 Jan 25;12 (1) :87. , URL: https://pubmed.ncbi.nlm.nih.gov/33494812/, *Zhang J, Tang J, Liu J, Yan B, Yan B, Huang M, Zhang Z, Wang L: Melatonin promotes heterotopic ossification through regulation of endothelial-mesenchymal transition in injured achilles tendons in rats. Front Cell Dev Biol. 2021 Feb 11;9:629274., URL: https://pubmed.ncbi.nlm.nih.gov/33644068/, *Chang Y, Ben Y, Li H, Xiong Y, Chen G, Hao J, Ma X, Gao X, Qiang P, Shimosawa T, Wang X, Yang F, Xu Q: Eplerenone prevents cardiac fibrosis by inhibiting angiogenesis in unilateral urinary obstruction rats. J Renin Angiotensin Aldosterone Syst. 2022 Sep 17;2022:1283729. , URL: https://pubmed.ncbi.nlm.nih.gov/36185701/

• Other References:

  *Zhu Y, Sun Y, Xie L, Jin K, Sheibani N, Greenberg DA: Hypoxic induction of endoglin via mitogen-activated protein kinases in mouse brain microvascular endothelial cells. Stroke. 2003 Oct;34 (10) :2483-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/12947156?ordin, *Li C, Issa R, Kumar P, Hampson IN, Lopez-Novoa JM, Bernabeu C, Kumar S: CD105 prevents apoptosis in hypoxic endothelial cells. J Cell Sci. 2003 Jul 1;116 (Pt 13) :2677-85., URL: https://www.ncbi.nlm.nih.gov/pubmed/12746487?ordin, *Guo B, Slevin M, Li C, Parameshwar S, Liu D, Kumar P, Bernabeu C, Kumar S: CD105 inhibits transforming growth factor-beta-Smad3 signalling. Anticancer Res. 2004 May-Jun;24 (3a) :1337-45., URL: https://www.ncbi.nlm.nih.gov/pubmed/15274293?ordin, *Warrington K, Hillarby MC, Li C, Letarte M, Kumar S: Functional role of CD105 in TGF-beta1 signalling in murine and human endothelial cells. Anticancer Res. 2005 May-Jun;25 (3B) :1851-64., URL: https://www.ncbi.nlm.nih.gov/pubmed/16158917?ordin, *Piao M, Tokunaga O: Significant expression of endoglin (CD105), TGFbeta-1 and TGFbeta R-2 in the atherosclerotic aorta: an immunohistological study. J Atheroscler Thromb. 2006 Apr;13 (2) :82-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/16733295?ordin

• Storage Conditions:

  Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

• Specificity:

  The antibody MEM-226 reacts with an extracellular epitope of CD105 (Endoglin), a 90 kDa type I homodimerizing membrane glycoprotein expressed on vascular endothelial cells (small and large vessels), activated monocytes and tissue macrophages, stromal cells of certain tissues including bone marrow, pre-B lymphocytes in fetal marrow and erythroid precursors in fetal and adult bone marrow; it is also present on syncytiotrophoblast on placenta throughout pregnancy.

• Applications Notes:

  6

• Species:

  Rat, Human

• CAS Number:

  9007-83-4

• Isotype:

  Mouse IgG2a