Anti-Hu CD5 Biotin

• Description:

  CD5 antigen (T1; 67 kDa) is a human cell surface T-lymphocyte single-chain transmembrane glycoprotein. CD5 is expressed on all mature T-lymphocytes, most of thymocytes, subset of B-lymphocytes and on many T-cell leukemias and lymphomas. It is a type I membrane glycoprotein whose extracellular region contains three scavenger receptor cysteine-rich (SRCR) domains. The CD5 is a signal transducing molecule whose cytoplasmic tail is devoid of any intrinsic catalytic activity. CD5 modulates signaling through the antigen-specific receptor complex (TCR and BCR) . CD5 crosslinking induces extracellular Ca++ mobilization, tyrosine phosphorylation of intracellular proteins and DAG production. Preliminary evidence shows protein associations with ZAP-70, p56lck, p59fyn, PC-PLC, etc. CD5 may serve as a dual receptor, giving either stimulatory or inhibitory signals depending both on the cell type and development stage. In thymocytes and B1a cells it seems to provide inhibitory signals, in peripheral mature T lymhocytes it acts as a costimulatory signal receptor. CD5 is the phenotypic marker of a B cell subpopulation involved in the production of autoreactive antibodies. Disease relevance: CD5 is a phenotypic marker for some B cell lymphoproliferative disorders (B-CLL, Hairy cell leukemia, etc.) . The CD5+ popuation is expanded in some autoimmune disorders (rheumatoid arthritis, etc.) . Herpes virus infections induce loss of CD5 expression in the expanded CD8+ human T cells.

• Certification:

  RUO

• Immunogen:

  Human acute lymphoblastic leukemia (ALL) T cells

• Target Antigen:

  CD5

• Clone:

  L17F12

• Applications:

  FC (QC tested), IP, WB, ICC

• Concentration:

  1 mg/mL

• Format:

  Biotin

• Buffer:

  Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• References & Citations:

  *Dunphy CH, Tang W: The value of CD64 expression in distinguishing acute myeloid leukemia with monocytic differentiation from other subtypes of acute myeloid leukemia: a flow cytometric analysis of 64 cases. Arch Pathol Lab Med. 2007 May;131 (5) :748-54., URL: http://www.ncbi.nlm.nih.gov/pubmed/17488160 , *Gong JZ, Lagoo AS, Peters D, Horvatinovich J, Benz P, Buckley PJ: Value of CD23 determination by flow cytometry in differentiating mantle cell lymphoma from chronic lymphocytic leukemia/small lymphocytic lymphoma. Am J Clin Pathol. 2001 Dec;116 (6) :893-7., URL: http://www.ncbi.nlm.nih.gov/pubmed/11764079 , *McAlister MS, Davis B, Pfuhl M, Driscoll PC: NMR analysis of the N-terminal SRCR domain of human CD5: engineering of a glycoprotein for superior characteristics in NMR experiments. Protein Eng. 1998 Oct;11 (10) :847-53., URL: https://www.ncbi.nlm.nih.gov/pubmed/9862202, *Shuster JJ, Falletta JM, Pullen DJ, Crist WM, Humphrey GB, Dowell BL, Wharam MD, Borowitz M: Prognostic factors in childhood T-cell acute lymphoblastic leukemia: a Pediatric Oncology Group study. Blood. 1990 Jan 1;75 (1) :166-73., URL: https://www.ncbi.nlm.nih.gov/pubmed/1688495, *Engleman EG, Warnke R, Fox RI, Dilley J, Benike CJ, Levy R: Studies of a human T lymphocyte antigen recognized by a monoclonal antibody. Proc Natl Acad Sci U S A. 1981 Mar;78 (3) :1791-5., URL: https://www.ncbi.nlm.nih.gov/pubmed/7015346

• Other References:

  *Freedman AS, Freeman G, Whitman J, Segil J, Daley J, Levine H, Nadler LM: Expression and regulation of CD5 on in vitro activated human B cells. Eur J Immunol. 1989 May;19 (5) :849-55. , URL: https://www.ncbi.nlm.nih.gov/pubmed/2472277?dopt=A, *Raman C: CD5, an important regulator of lymphocyte selection and immune tolerance. Immunol Res. 2002;26 (1-3) :255-63., URL: https://pubmed.ncbi.nlm.nih.gov/12403363/

• Storage Conditions:

  Store at 2-8°C. Do not freeze.

• Specificity:

  The mouse monoclonal antibody L17F12 reacts with an extracellular epitope of CD5, a 67kDa single-chain transmembrane glycoprotein expressed on mature T lymphocytes, most of thymocytes and B lymphocytes subset (B-1a lymphocytes) .

• Applications Notes:

  Flow cytometry: Recommended dilution: 1-4 μg/ml.

• Species:

  Human

• CAS Number:

  9007-83-4

• Isotype:

  Mouse IgG2a kappa