Rat Ig and IgG subclasses standard Serum

• Background:

  This standard is prepared by pooling serum obtained from a large number rats with the following assigned values: IgG1--1.68 mg/ml. IgG2a--3.36 mg/ml. IgG2b--7.66 mg/ml. IgG2c--0.50 mg/ml. IgA--2.43 mg/ml. IgM--1.31 mg/ml. The determination of the concentration of each of the four subclasses was carried out by single radial immunodiffusion (RID, Mancini) in agar gel Nordic Nr. 2 using subclass-specific polyclonal antisera and purified rat immunoglobulins as primary standards. Rat standard serum NOR-03 is intended for use as a calibrator to prepare standard curves in quantitative determinations of the immunoglobulins IgA and IgM and IgG subclasses.

• Type:

  Serum

• Field of Research:

  Immunology, Immunology

• Assay Principle:

  The product is shipped lyophilized at ambient temperature. The lyophilized content is reconstituted by adding 0.5 mL sterile water, mixed gently by inversion until complete dissolution is obtained. Allow to stand at ambient temperature for 5-10 minutes to reach equilibrium. Reconstituted serum may be divided into small aliquots, frozen at or below -20°C.

• Form:

  Delipidated, lyophilized serum; No preservative added

• Precautions:

  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.