Anti-Phospho-Glycogen Synthase 1 S641 GYS1 Antibody

• Background:

  Anti-Glycogen Synthase 1 pS641 antibody is validated by IHC, Western Blot and ELISA. Human muscle glycogen synthase (GS) is responsible for the biosynthesis of glycogen from phosphorylated glucose units. Mammalian liver and muscle contain GS consisting of four subunits with a total molecular weight of 360,000. GS is subject to regulation through both allosteric and covalent modification and occurs in two forms: the phosphorylated inactive form, and the dephosphorylated active form. GS is inactivated by the serine/threonine kinase called glycogen synthase kinase-3 that mainly functions to phosphorylate muscle glycogen synthase. This antibody is specific for the phosphorylated form of GS at S641. Phosphorylation of GS at S641 has been associated with Antiphospholipid Antibody Syndrome.

• Description:

  Boster Bio Anti-Phospho-Glycogen Synthase 1 S641 GYS1 Antibody (Catalog # P03512) . Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse.

• Synonyms:

  Muscle glycogen synthase pS641, glycogen synthase pS641, Glycogen, Glycogen synthase 1 (muscle), Glycogen synthase 1, Glycogen synthase1, GYS 1, GYS-1, GYS1, Starch synthase muscle

• Gene Name:

  GYS1

• UniProt:

  P13807

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  Human Muscle Glycogen Synthase phospho peptide corresponding to pS641 region of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH) .

• Clonality:

  Polyclonal

• Applications:

  ELISA, IHC, WB

• Field of Research:

  Atherosclerosis, Cancer, Cancer Metabolism, Carbohydrate Metabolism, Cardiovascular, Diabetes, Diabetes-associated, Energy Metabolism, Energy Transfer Pathways, Heart Disease, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Metabolism Of Carbohydrates, Obesity, Pathways and Processes, Signal Transduction

• Purification:

  Phospho Glycogen Synthase pS641 antibody is directed against human muscle glycogen synthase. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. This phospho specific polyclonal antibody is specific for phosphorylated pS641 of human muscle glycogen synthase. Reactivity with non-phosphorylated human muscle glycogen synthase is less than 1% by ELISA. Cross-reactivity with muscle glycogen synthase occurs in mouse tissue. Reactivity with muscle glycogen synthase from other sources has not been determined.

• Concentration:

  0.95 mg/mL by UV absorbance at 280 nm

• Form:

  Liquid (sterile filtered)

• Function:

  Transfers the glycosyl residue from UDP-Glc to the non- reducing end of alpha-1,4-glucan.

• Molecular Weight:

  83786 MW

• Shipping Conditions:

  Due to dry ice shipping issues, this product is temporarily not available to distributors outside of the United States.

• Storage Conditions:

  Store Phospho GYS1 antibody at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

• Fragment:

  IgG

• Applications Notes:

  This phospho specific polyclonal antibody was tested by immunohistochemistry, immunoblotting, and ELISA. By ELISA the antibody was found to be reactive with the phosphorylated form of the immunizing peptide and minimally reactive with the non-phosphorylated form of the immunizing peptide. Immunoblotting will detect human and mouse muscle glycogen synthase. Although not tested, this antibody is likely functional in immunoprecipitation.

• Other Gene Names:

  Glycogen [starch] synthase, muscle