Anti-TLE1 Rabbit Monoclonal Antibody

• Description:

  Boster Bio Anti-TLE1 Rabbit Monoclonal Antibody catalog # M02460. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

• Synonyms:

  Major prion protein; PrP; ASCR; PrP27-30; PrP33-35C; CD230; PRNP; ALTPRP, PRIP, PRP

• Gene Name:

  Major prion protein

• UniProt:

  Q04724

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  A synthesized peptide derived from human TLE 1 Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling.

• Clonality:

  Monoclonal

• Clone:

  Clone: AFEA-20

• Tissue Specificity:

  Ubiquitous.

• Applications:

  WB, IHC, ICC, IF

• Field of Research:

  Hematopoietic Progenitors, Neurodegenerative Disease, Neurology Process, Neuroscience, Stem Cells, Surface Molecules

• Purification:

  Affinity-chromatography

• Concentration:

  Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

• Form:

  Liquid

• Reconstitution:

  Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

• Function:

  May play a role in neuronal development and synaptic plasticity. May be required for neuronal myelin sheath maintenance. May play a role in iron uptake and iron homeostasis. Soluble oligomers are toxic to cultured neuroblastoma cells and induce apoptosis (in vitro) . Association with GPC1 (via its heparan sulfate chains) targets PRNP to lipid rafts. Also provides Cu (2+) or ZN (2+) for the ascorbate-mediated GPC1 deaminase degradation of its heparan sulfate side chains (By similarity) . .

• Storage Conditions:

  Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

• Fragment:

  Rabbit IgG

• Applications Notes:

  WB 1:500-1:2000
  IHC 1:50-1:200
  ICC/IF 1:50-1:200

• Other Gene Names:

  TLE1

• Subcellular Location:

  Cell membrane; Lipid-anchor, GPI-anchor. Golgi apparatus. Targeted to lipid rafts via association with the heparan sulfate chains of GPC1. Colocates, in the presence of CU (2+), to vesicles in para- and perinuclear regions, where both proteins undergo internalization. Heparin displaces PRNP from lipid rafts and promotes endocytosis.

• Protein Name:

  T-cell surface glycoprotein CD3 zeta chain