Anti-Ubiquitin C-terminal Hydrolase 1 (UCHL1) Monoclonal Antibody

• Description:

  Boster Bio Anti-Ubiquitin C-terminal Hydrolase 1 (UCHL1) Monoclonal Antibody catalog # M01018-3. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Bovine, Equine, Human, Mouse, Pig, Rat.

• Synonyms:

  Ubiquitin carboxyl-terminal hydrolase isozyme L1; UCH-L1;3.4.19.12;6.-.-.-; Neuron cytoplasmic protein 9.5; PGP 9.5; PGP9.5; Ubiquitin thioesterase L1; UCHL1

• Gene Name:

  UCHL1

• UniProt:

  P09936

• Host:

  Mouse

• Reactivity:

  Human, Rat, Mouse, Bovine, Pig, Equine

• Immunogen:

  Recombinant full length human UCHL1 purified from E. coli.

• Clonality:

  Monoclonal

• Tissue Specificity:

  Found in neuronal cell bodies and processes throughout the neocortex (at protein level) . Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients. .

• Applications:

  IF, IHC, ICC, WB

• Concentration:

  0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

• Dilution:

  Western blot: 1:20,000. ICC/IF and IHC: 1:2,000.

• Function:

  Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity. .

• Molecular Weight:

  24824 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

• Other Gene Names:

  Ubiquitin carboxyl-terminal hydrolase isozyme L1

• Subcellular Location:

  Cytoplasm . Endoplasmic reticulum membrane ; Lipid-anchor . About 30% of total UCHL1 is associated with membranes in brain.