Human B7-1/CD80 ELISA Kit PicoKine®

• Background:

  The protein CD80 (Cluster of Differentiation 80) is a molecule found on activated B cells and monocytes which provides a costimulatory signal necessary for T cell activation and survival. It is also known as B7-1. The cDNA for B7-1 predicts a type I membrane protein, i.e., one synthesized with a signal peptide that is cleaved upon translocation across the endoplasmic membrane. The protein is predicted to contain 2 extracellular domains structurally similar to those of Ig, a hydrophobic transmembrane region, and a short cytoplasmic domain. The CD80 and CD86 genes encode B7-1 and B7-2, respectively, which are structurally similar members of the immunoglobulin superfamily expressed on a variety of hematopoietic cell types. B7-1 and B7-2 provide a costimulatory signal to T cells by interacting with CD28 and CTLA4.

• Description:

  Human B7-1/CD80 ELISA Kit PicoKine® (96 Tests) . Quantitate Human CD80 in cell culture supernatants and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

• Synonyms:

  T-lymphocyte activation antigen CD80; Activation B7-1 antigen; BB1; CTLA-4 counter-receptor B7.1; B7; CD80; CD80; CD28LG, CD28LG1, LAB7

• Gene Name:

  T-lymphocyte activation antigen CD80

• UniProt:

  P33681

• Reactivity:

  Human

• Cross Reactivity:

  There is no detectable cross-reactivity with other relevant proteins.

• Immunogen:

  Expression system for standard: NS0; Immunogen sequence: V35-N242

• Tissue Specificity:

  Expressed on activated B-cells, macrophages and dendritic cells.

• Applications:

  ELISA

• Field of Research:

  Adaptive Immunity, B Cells, Hematopoietic Progenitors, Immunology, Lymphoid, Myeloid, Stem Cells

• Sample Type:

  Cell culture supernatants and serum.

• Detection Range:

  62.5 pg/ml - 4,000 pg/ml

• Sensitivity:

  <10 pg/ml

• Function:

  Involved in the costimulatory signal essential for T- lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. .

• Storage Conditions:

  Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

• Other Gene Names:

  CD80

• Subcellular Location:

  Membrane; Single-pass type I membrane protein.

• Sequence Similarities:

  Contains 1 Ig-like C2-type (immunoglobulin-like) domain.

• Protein Name:

  T-lymphocyte activation antigen CD80