5-bromo-4-chloro-3-indolyl β-D-galactopyranoside

• Description:

  5-bromo-4-chloro-3-indolyl β-D-galactopyranoside_x000D_ Catalog number: B2012172_x000D_ Lot number: Batch Dependent_x000D_ Expiration Date: Batch dependent_x000D_ Amount: 50 mg_x000D_ Molecular Weight or Concentration: 408.63 g/mol_x000D_ Supplied as: Powder_x000D_ Applications: molecular tool for various biochemical applications_x000D_ Storage: -20°C_x000D_ Keywords: 5-Bromo-4-chloro-3-(β-D-galactopyranosyloxy)indole, X-Gal_x000D_ Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um._x000D_ _x000D_ References:_x000D_ 1: Itahana K, Campisi J, Dimri GP. Methods to detect biomarkers of cellular senescence: the senescence-associated beta-galactosidase assay Methods Mol Biol. 2007;371:21-31._x000D_ 2: Gary RK, Kindell SM. Quantitative assay of senescence-associated beta-galactosidase activity in mammalian cell extracts Anal Biochem. 2005 Aug 15;343(2):329-34._x000D_ 3: Aspelund A, Antila S, Proulx ST, Karlsen TV, Karaman S, Detmar M, Wiig H, Alitalo K. A dural lymphatic vascular system that drains brain interstitial fluid and macromolecules J Exp Med. 2015 Jun 29;212(7):991-9._x000D_ 4: Green MR, Sambrook J. Screening Bacterial Colonies Using X-Gal and IPTG: α-Complementation Cold Spring Harb Protoc. 2019 Dec 2;2019(12)._x000D_ 5: Lozano-Gerona J, García-Otín ÁL. ImageJ-based semiautomatic method to analyze senescence in cell culture Anal Biochem. 2018 Feb 15;543:30-32._x000D_ 6: Rodig SJ. Detecting β-Galactosidase-Labeled Cells Cold Spring Harb Protoc. 2020 May 1;2020(5):099739._x000D_ 7: Arakawa H, Ikegami T, Maeda M, Tsuji A. Chemiluminescent enzyme immunoassay for alpha-fetoprotein using beta-D-galactosidase as label and 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside as substrate J Biolumin Chemilumin. 1993 May-Jun;8(3):135-9._x000D_ 8: Ley A, Barr S, Fredenburgh D, Taylor M, Walker N. Use of 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside for the isolation of beta-galactosidase-positive bacteria from municipal water supplies Can J Microbiol. 1993 Sep;39(9):821-5._x000D_ 9: Kobayashi S, Okada M. A double staining technique using 5-bromo,4-chloro-3-indolyl-beta-D-galactopyranoside (X-gal) and immunoperoxidase in whole Drosophila embryos Biotech Histochem. 1993 Jul;68(4):237-9._x000D_ 10: Krämer MS, Feil R, Schmidt H. Analysis of Gene Expression Using lacZ Reporter Mouse Lines Methods Mol Biol. 2021;2224:29-45. _x000D_ _x000D_ Products Related to 5-bromo-4-chloro-3-indolyl β-D-galactopyranoside can be found at Substrates

• Short Description:

  Catalog Number: B2012172 (50 mg)

• Weight:

  0.15

• Length:

  2

• Width:

  0.5

• Height:

  0.5

• Height :

  0.5