Anti-Mouse GM-CSF (MP1-22E9) In Vivo Antibody - Low Endotoxin

• Background:

  Granulocyte-macrophage colony-stimulating factor, often abbreviated to GM-CSF is a pleiotropic cytokine that controls the production and function of blood cells. GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes. GM-CSF has a critical role in regulation of surfactant homeostasis and alveolar macrophage innate immune functions in the lung.

• Specifications:

  Anti-GM-CSF In Vivo Antibody - Low Endotoxin (MP1-22E9) recognizes an epitope on Mouse GM-CSF

• Product Name Alternative:

  Csf2, Granulocyte-macrophage colony-stimulating factor, Colony-stimulating factor, CSF, Csfgm

• UniProt:

  P01587

• Host:

  Rat

• Species Reactivity:

  Mouse

• Target:

  GM-CSF

• Isotype:

  IgG2a

• Clone:

  MP1-22E9

• Applications:

  Western Blot, ELISA, ELISPOT, Blocking, Immunocytochemistry, IHC (Frozen)

• Purification Method:

  This monoclonal antibody was purified using Protein G

• Concentration:

  1.0 - 5.0 mg/mL

• Endotoxin:

  ≤ 1.0 EU/mg as determined by the LAL method

• Purity:

  >95% by SDS-PAGE and HPLC

• Buffer:

  ICH3001-100mL ICH3002-100mL ICH3003-100mL

• Shipping Conditions:

  Blue ice

• Storage Conditions:

  Anti-GM-CSF In Vivo Antibody - Low Endotoxin (MP1-22E9) is lyophilized and can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

• Formulation:

  0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

• Aggregation:

  Aggregation level ≤ 5%

• Applications Notes:

  Western Blotting: To detect Mouse GM-CSF this monoclonal antibody can be used at a concentration of 1-2 µg/ml.Each investigator should determine their own optimal working dilution for specific applications.

• Isotype Control:

  https://www.ichor.bio/product/Rat-IgG2a-In-Vivo-Isotype-Control-Low-Endotoxin-1-1-ICH2244

• CAS Number:

  9007-83-4