Anti-Hu TCR Valpha24-Jalpha18 PE

• Description:

  TCR alpha V24-J18 chain is a marker of invariant NK T cells (iNKT) . This chain pairs mostly with TCR beta V11 chain, and together they interact with glycolipide-loaded CD1d. This interaction results in iNKT activation and cytokine production (IFN gamma, IL-4, IL-13), which takes important part in immune regulation and host response to pathogens and cancer (support of both Th1 and Th2 way), although iNKT cells represent less than 1% of total peripheral T cells.

• Certification:

  RUO

• Target Antigen:

  TCR Valpha24-Jalpha18

• Clone:

  6B11

• Applications:

  FC (QC tested)

• Format:

  PE

• Buffer:

  Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• References & Citations:

  *Montoya CJ, Pollard D, Martinson J, Kumari K, Wasserfall C, Mulder CB, Rugeles MT, Atkinson MA, Landay AL, Wilson SB: Characterization of human invariant natural killer T subsets in health and disease using a novel invariant natural killer T cell-clonotypic monoclonal antibody, 6B11. Immunology. 2007 Sep;122 (1) :1-14., URL: https://pubmed.ncbi.nlm.nih.gov/17662044/, *Vyth-Dreese FA, Sein J, van de Kasteele W, Dellemijn TA, van den Bogaard C, Nooijen WJ, de Gast GC, Haanen JB, Bex A: Lack of anti-tumour reactivity despite enhanced numbers of circulating natural killer T cells in two patients with metastatic renal cell carcinoma. Clin Exp Immunol. 2010 Dec;162 (3) :447-59., URL: https://pubmed.ncbi.nlm.nih.gov/20942806/, *Krovi SH, Gapin L: Invariant natural killer T cell subsets - more than just developmental intermediates. Front Immunol. 2018 Jun 20;9:1393., URL: https://pubmed.ncbi.nlm.nih.gov/29973936/, *Chamoto K, Guo T, Scally SW, Kagoya Y, Anczurowski M, Wang CH, Rahman MA, Saso K, Butler MO, Chiu PP, Julien JP, Hirano N: Key residues at third CDR3β position impact structure and antigen recognition of human invariant NK TCRs. J Immunol. 2017 Feb 1;198 (3) :1056-1065. , URL: https://pubmed.ncbi.nlm.nih.gov/28003379/

• Storage Conditions:

  Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

• Specificity:

  The mouse monoclonal antibody 6B11 recognizes an extracellular epitope within V alpha 24 J alpha 18 TCR, which is expressed by invariant NK T cells (iNKT) .

• Applications Notes:

  6

• Species:

  Human, Non-human primates

• CAS Number:

  9007-83-4

• Isotype:

  Mouse IgG1 kappa