Human CD5 Protein, His Tag

• Background:

  T-cell surface glycoprotein CD5 is also known as Lymphocyte antigen T1/Leu-1 and LEU1, which is phosphorylated on tyrosine residues by LYN, so CD5 can create binding sites for PTPN6/SHP-1.CD5 may act as a receptor in regulating T-cell proliferation. CD5 is expressed at various developmental and activation stages on human B cells.CD5 is a well established negative regulator of TCR and BCR signalling.CD5-positive cells may also prevent the emergence of autoimmunity by provision of cytokines such as IL-10. Development, selection and function of different B- and T-cell subsets or their preferential survival may be directly or indirectly dependent on different glycan structures associated with CD5 or CD5-like molecules.

• Description:

  Human CD5, His Tag (CD5-H52H5) is expressed from human 293 cells (HEK293) . It contains AA Arg 25 - Pro 372 (Accession # NP_055022) .

• Specifications:

  This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 40.5 kDa. The protein migrates as 45-53 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

• Accession Number:

  NP_055022

• Expression Region:

  Arg 25 - Pro 372

• Host:

  HEK293

• Target:

  CD5

• Conjugation:

  Unconjugated

• Tag:

  C-10xHis

• Source:

  Human

• Stability:

  ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

• Endotoxin:

  1.0 EU per μg

• Purity:

  95%

• Bioactivity:

  Immobilized Human CD5, His Tag (Cat. No. CD5-H52H5) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CD5 Antibody, Mouse IgG1 (2C8A7) with a linear range of 0.06-4 ng/mL (QC tested).

• Format:

  Powder

• Buffer:

  PBS, pH7.4

• Additives:

  Trehalose

• Molecular Weight:

  40.5 kDa

• Shipping Conditions:

  RT

• Storage Conditions:

  -20°C

• Package Size:

  100ug*1

• Species:

  Human