VGLUT2 Antibody

• Background:

  The ATP-dependent, chloride-sensitive vesicular glutamate transporters (VGLUT) include BNPI (VGLUT1), VGLUT2 (DNPI) and VGLUT3. The brain expresses BNPI (brain specific Na+-dependent inorganic phosphate (Pi) cotransporter) and VGLUT2 in a complementary fashion. The telencephalic regions express BNPI, whereas the lower brainstem and diencephalic regions express VLGUT2. Rat pinealocytes express both BNPI and VGLUT2. The striatum, hippocampus, cerebral cortex and raphe nuclei express VGLUT3 in a small number of neurons. Pancreatic α and β cells express BNPI and VGLUT2 in response to glucose concentrations. Human VGLUT3 shares a 72% sequence homology with VLGUT2 and BNPI.

• Description:

  Mouse Anti-Rat VGLUT2 Monoclonal IgG1

• Specifications:

  Detects ~60kDa.

• Product Name Alternative:

  Differentiation associated BNPI Antibody, DNPI Antibody, SLC17A6 Antibody, Solute carrier family 17 member 6 Antibody, Vesicular glutamate transporter 2 Antibody, Differentiation associated Na dependent inorganic phosphate cotransporter Antibody, Solute carrier family 17 (Sodium dependent inorganic phosphate cotransporter) member 6 Antibody, Differentiation associated Na(+) dependent inorganic phosphate cotransporter Antibody, Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter Antibody, Sodium dependent inorganic phosphate cotransporter Antibody

• Synonyms:

  VGLUT2 Antibody, Clone N29/29

• UNSPSC:

  12352203

• Gene ID:

  84487

• Swiss Prot:

  Q9JI12

• Accession Number:

  NP_445879.1

• Cellular Locus:

  Cytoplasmic Vesicle | Secretory Vesicle | Synaptic Vesicle Membrane | Membrane | Cell Junction | Synapse

• Host:

  Mouse

• Species Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Fusion protein amino acids 501-582 (cytoplasmic C-terminus) of rat VGlut2

• Target:

  VGLUT2

• Clonality:

  Monoclonal

• Isotype:

  IgG1

• Clone:

  N29/29 (Formerly sold as S29-29)

• Conjugation:

  Unconjugated

• Validated Applications:

  WB, IHC, ICC/IF

• Purification:

  Protein G Purified

• Limit Of Detection:

  1 µg/ml of SMC-395 was sufficient for detection of VGLut2 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

• Concentration:

  1 mg/ml

• Dilution:

  WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

• Weight:

  0.012

• Buffer:

  PBS pH7.4, 50% glycerol, 0.09% sodium azide

• Precautions:

  Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

• References & Citations:

  1. Aihara Y., et al. (2000) J Neurochem. 74: 2622-2625. 2. Kaneko T., and Fujiyama F. (2002) Neurosci Res. 42: 243-250. 3. Gras C., et al. (2002) J Neurosci. 22: 5442-5451. 4. Takamori S., Malherbe P., Broger C., and Jahn R. (2002) EMBO Rep. 3: 798-803. 5. Morimoto R., Hayashi M., Yatsushiro S., Otsuka M., Yamamoto A., and Moriyama Y. (2003) J Neurochem. 84: 382-391. 6. Bai L., Zhang X., and Ghishan F.K. (2003) Am. J. Physiol. Gastrointest. Liver Physiol. 284: G808-814.

• CAS Number:

  9007-83-4