CaMKII (Alpha-Specific) Antibody

• Background:

  CaMKII is an important member of the calcium/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling (1, 2). CaMKII is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites and the soma of the neuron. The CaMKII that is found in the neurons consist of two subunits of 52 (termed alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains, as well as an ATP-binding domain, and a consensus phosphorylation site (3-7). The binding of Ca2+/calmodulin to its regulatory domain releases its auto inhibitory effect and activates the kinase (8). This kinase activation results in autophosphorylation at threonine 286 (8). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. Whereas PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286, PKA (protein kinase A) prevents this dephosphorylation (9). Autophosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities (10-12).

• Description:

  Mouse Anti-Rat CaMKII (Alpha-Specific) Monoclonal IgG1

• Specifications:

  Detects ~50-60kDa. Recognizes both phosphorylated and non-phosphorylated forms.

• Product Name Alternative:

  CamK2 Antibody, CamK2A Antibody, CamK2B Antibody, CamK2D Antibody, CamK2G Antibody, CAMKA Antibody

• Synonyms:

  CaMKII Antibody, Clone 6G9

• UNSPSC:

  12352203

• Gene ID:

  12322

• Swiss Prot:

  P11798

• Accession Number:

  NP_033922.1

• Cellular Locus:

  Cytoplasm | Mitochondrion | Nucleus | Cell Junction | Synapse | Presynaptic Cell Membrane

• Host:

  Mouse

• Species Reactivity:

  Human, Mouse, Rat, Bovine

• Immunogen:

  Partially purified rat CaMKII

• Target:

  CaMKII

• Clonality:

  Monoclonal

• Isotype:

  IgG1

• Clone:

  6G9

• Conjugation:

  Unconjugated

• Validated Applications:

  WB, IHC, ICC/IF, IP, ELISA, RIA

• Purification:

  Protein G Purified

• Limit Of Detection:

  0.1 µg/ml was sufficient for detection of CamKII in 20 µg rat brain tissue extract by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:AP as the secondary.

• Concentration:

  1 mg/ml

• Dilution:

  WB (1:10000), IHC (1:2000), ICC/IF (1:50); optimal dilutions for assays should be determined by the user.

• Weight:

  0.012

• Buffer:

  PBS pH7.4, 50% glycerol, 0.09% sodium azide

• Precautions:

  Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

• References & Citations:

  1. Hughes K. et al. (2001) J. Biol. Chem. 276: 36008–36013. 2. Barria A. et al. (1997) Science 276: 2042–2045. 3. Bennet M.K. and Kennedy M.B. (1987) Proc. Natl. Acad. Sci. U.S.A. 84: 1794-1798. 4. Broke L., Srinivasan M. and Schulman H. (1995) J. Neurosci. 15: 6797-6808. 5. Nghiem P., Saati S. M., Martens C. L., Gardner P. and Schulman H. (1993) J. Biol. Chem. 268: 5471-5479. 6. Edman C.F. and Schulman H. (1994) Biochem. Biophys. Acta 1221: 90-102. 7. Tombes R.M. and Krystal G.W., (1997) Biochem. Biophys. Acta 13555: 281-292. 8. Means A.R. (2000) Mol. Endocrinol. 14: 4–12. 9. Makhinson M. et al. (1999) J. Neurosci. 19: 2500–2510. 10. Strack S. and Colbran R.J. (1998) J. Biol. Chem. 273: 20689-20692. 11. Leonard S.A., Lim I.A., Hemsworth D.E., Horne M.C. and Hell J.W. (1999) Proc. Natl. Acad. Sci. U.S.A. 96: 3239-3244. 12. Shen K. and Meyer Y. (1999) Science 284: 162-167.

• CAS Number:

  9007-83-4