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2xHieff™ Ultra-Rapid II HotStart PCR Master Mix

CAT: 1015-10167ES03Size: 1 mLDry Ice: YesHazardous: No
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CAT#:1015-10167ES03Size:1 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
2×Hieff™ Ultra-Rapid II HotStart PCR Master Mix contains heat-stabilized Taq DNA Polymerase modified with antibodies, adds strong elongation factor and optimized buffer system, and has super high amplification efficiency. It is very suitable for PCR amplification of most colonies such as E. coli, Agrobacterium, and yeast. The extension speed of amplification of complex templates within 3 kb can reach 1 sec/kb, 3-6 kb to 3 sec/kb, 6-10 kb to 5 sec/kb and 10 kb to 10 sec/kb; the amplification speed of simple templates such as plasmid within 6 kb can reach 1 sec/kb, which can greatly save PCR reaction time. Meanwhile, Mix contains dNTPs, Mg2+, and it can be amplified only with adding primers and template. In addition, Mix contains red tracer dye, which can be used by electrophoresis directly after the end of the reaction. The protective agent added to the system enables the product to maintain a stable activity after repeated freezing and thawing. The 3′ end of the PCR product bands A and can be easily cloned into the T vector.
Specifications
2× Hieff™ Ultra-Rapid II HotStart PCR Master Mix is supplied as a 2× concentrated PCR master mix containing antibody-modified thermostable Taq DNA polymerase, dNTPs, Mg²⁺ and optimized reaction components. The product is designed for rapid DNA amplification and supports applications including colony PCR, rapid PCR, genotype identification and amplification of complex templates. It reports amplification capabilities for targets with GC content up to 70% and fragment lengths up to 10 kb in its product positioning, with rapid extension performance reported at up to 1 sec/kb under applicable conditions. The product is supplied in 1 mL and 5 × 1 mL formats and is stored at −25 to −15°C for 2 years.
Field of Research
Molecular Biology
Features & Benefits
  • 2× ready-to-use PCR master mix
  • Antibody-modified thermostable Taq DNA polymerase
  • Strong extension factor
  • Optimized PCR buffer system
  • Rapid amplification
  • Suitable for colony PCR
  • Suitable for genotype identification
  • Suitable for complex templates
  • Supports high-GC templates up to 70% according to manufacturer information
  • Target amplification up to 10 kb in the manufacturer's product positioning
  • Rapid extension performance of up to 1 sec/kb under applicable conditions
  • Stable for 2 years at −25 to −15°C
Shipping Conditions
Dry ice
Package Size
1 mL

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