Human APP / Abeta40 Protein, His Tag

• Background:

  Amyloid precursor protein (APP) is a type I integral membrane protein ubiquitously expressed in many tissues and concentrated in the synapses of neurons. It has three predominant splice variants: APP695, APP751, and APP770. The majority of APP is cleaved at the plasma membrane by the α-secretase in the non-amyloidogenic pathway. The amyloidogenic pathway starts with β-secretase cleavage by BACE1 on the N-terminal part of the Aβ domain, releasing sAPPβ from a membrane-anchored fragment named βCTF or C99, which is subsequently cleaved by γ-secretase to release Aβ.

• Description:

  Human Abeta40, His Tag (APP-H51H7) is expressed from E. coli cells. It contains AA Asp 672 - Val 711 (Accession # P05067-1).

• Specifications:

  This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 6.3 kDa. The protein migrates as 11 kDa under reducing (R) condition (SDS-PAGE).

• Expression Region:

  Asp 672 - Val 711

• Host:

  E. coli

• Target:

  APP

• Conjugation:

  Unconjugated

• Tag:

  C-10×His

• Source:

  Human

• Stability:

  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.

• Endotoxin:

  1.0 EU per μg

• Purity:

  95%

• Bioactivity:

  Immobilized Human Abeta40, His Tag (Cat. No. APP-H51H7) at 5 μg/mL (100 μL/well) can bind Aducanumab with a linear range of 0.2-2 ng/mL (QC tested).

• Format:

  Powder

• Buffer:

  PBS, 0.2 M Arginine, pH7.4

• Additives:

  Trehalose

• Molecular Weight:

  6.3 kDa

• Shipping Conditions:

  Room Temperature

• Storage Conditions:

  -20°C