QuicKey Pro Human VCAM-1/CD106 (Vascular Cell Adhesion Molecule 1) ELISA Kit

• Abbreviation:

  VCAM-1

• UniProt:

  P19320

• Cross Reactivity:

  This kit recognizes VCAM-1 in samples.No significant cross-reactivity or interference between VCAM-1 and analogues was observed.

• Target:

  VCAM1; CD106; INCAM-100; vascular cell adhesion molecule 1

• Type:

  Sandwich-ELISA

• Applications:

  ELISA

• Field of Research:

  Cancer; Cardiovascular; Neuroscience; Signal transduction

• Assay Principle:

  This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human VCAM-1/CD106. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human VCAM-1/CD106 are added to the micro ELISA plate wells. Human VCAM-1/CD106 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human VCAM-1/CD106.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human VCAM-1/CD106. The concentration of Human VCAM-1/CD106 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  1 h 30 min

• Sample Type:

  Serum, plasma and other biological fluids

• Detection Range:

  0.78-50 ng/mL

• Precision:

  Both intra-CV and inter-CV are < 10%.

• Recovery:

  80%-120%

• Sensitivity:

  0.47 ng/mL

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  2-8°C,6 months

• Detection Instrument:

  Microplate reader

• Species:

  Human

• Recommended Usage:

  This ELISA kit applies to the in vitro quantitative determination of Human VCAM-1/CD106 concentrations in serum, plasma and other biological fluids.