Biotinylated Mouse PSMA / FOLH1 Protein, His, Avitag™

• Background:

  Prostate-specific membrane antigen (PSMA) is also known as Folate hydrolase 1 (FOLH1), Glutamate carboxypeptidase 2 (GCP2), N-acetylated-alpha-linked acidic dipeptidase I (ALAD1), which belongs to the peptidase M28 family and M28B subfamily. FOLH1 / PSMA is stable at pH greater than 6.5. FOLH1 / PSMA is a type II transmembrane zinc metallopeptidase that is most highly expressed in the nervous system, prostate, kidney, and small intestine. FOLH1 / GCP-2 is homodimer and binds 2 zinc ions per subunit, and required for ALADase activity. The catalytic activity of PSMA involved in releasing of an unsubstituted, C-terminal glutamyl residue, typically from Ac-Asp-Glu or folylpoly – gamma - glutamates. FOLH1 / GCP-2 / PSMA has both folate hydrolase and N – acetylated – alpha – linked - acidic dipeptidase (ALADase) activity and has a preference for tri-alpha-glutamate peptides. GCP-2 / PSMA involved in prostate tumor progression and also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC.

• Description:

  Biotinylated Mouse PSMA Protein, His, Avitag (PSA-M82Q4) is expressed from human 293 cells (HEK293) . It contains AA Ile 44 - Ala 752 (Accession # O35409-1) .

• Specifications:

  This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 83.2 kDa. The protein migrates as 95-120 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

• Accession Number:

  O35409-1

• Expression Region:

  Ile 44 - Ala 752

• Host:

  HEK293

• Target:

  PSMA

• Conjugation:

  Biotin-labeled

• Tag:

  N-10xHis & N-Avi

• Source:

  Mouse

• Stability:

  ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

• Endotoxin:

  1.0 EU per μg

• Purity:

  95%

• Format:

  Liquid

• Buffer:

  25 mM MES, 500 mM NaCl, pH6.5

• Additives:

  Glycerol

• Molecular Weight:

  83.2 kDa

• Shipping Conditions:

  Dry ice

• Storage Conditions:

  -70°C

• Package Size:

  25ug*1

• Species:

  Mouse