Glyoxal (High Density) 6% Agarose Beads, Crosslinked

• Description:

  Biomolecules can be immobilized by binding them to a support under conditions that will then determine the characteristics of the complex. Immobilization is a technique that binds a ligand (enzyme, antibody, affinity proteins like Protein A or Protein G) to a support, giving high stability and making it easier to reuse the immobilized molecule.Glyoxal beads allow a covalent binding of agarose to lysine amino groups (similar orientation to CNBr) . Glyoxal agarose beads are ready to use and require no activation. The resin has a high amount of aldehydes groups (40-60 µmoles Glyoxal / ml gel) providing a very good option to conjugate affinity ligands improving the stability of the ligand.The choice of Glyoxal or Aminoethyl will depend on the biomolecule to be immobilized, the accessibility of the reactive groups, and the direction/ orientation required for the binding to the support.Glyoxal 6% agarose beads are suitable for use with FPLC, but with pressuresno more than 20 kPa. Too much pressure or too fast of a flow rate will result in diminished performance.Choosing High and Low Density Agarose BeadsHigh Density ResinLow Density ResinHigh CrosslinkingLow CrosslinkingBest for smaller proteinsBest for larger proteins (e.g. antibodies) Best for repeated use or FPLC systemsBest for batch incubations or gravity flowSmall poresLarge poresStronger covalent binding of ligands improves ligand stabilityWeaker covalent binding improves ligand mobility after immobilization

• Stability:

  Stable in all commonly used reagents.

• Activity:

  40-60 (μmol glyoxyl/ml gel)

• Capacity:

  20 - 25 (mg BSA/ml gel)

• Dimensions:

  Spherical, Standard: ~50-150 μm (approx.)

• Storage Conditions:

  Store at 4°C. Do not freeze.

• Notes:

  Store at 4°C. Do NOT freeze.

• Identity:

  G-303