Recombinant LIMK1 Monoclonal Antibody

• Background:

  LIM kinases (LIMK1 and LIMK2) are serine/threonine kinases that have two zinc finger motifs, known as LIM motifs, in their amino-terminal regulatory domains. LIM kinases are involved in actin cytoskeletal regulation downstream of Rho-family GTPases, PAKs, and ROCK. PAK1 and ROCK phosphorylate LIMK1 or LIMK2 at the conserved Thr508 or Thr505 residues in the activation loop, increasing LIMK activity. Activated LIM kinases inhibit the actin depolymerization activity of cofilin by phosphorylation at the amino-terminal Ser3 residue of cofilin.

• Abbreviation:

  LIMK1

• UniProt:

  P53667

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Peptide. This information is proprietary to PTMab.

• Target:

  EC 2.7.1.; LIM domain kinase; LIMK1; LIMK; LIMK-1; EC 2.7.1.37; LIM domain containing protein kinase; LIM domain kinase 1; LIM kinase; LIM motif containing protein kinase; LIMK 1

• Clonality:

  Monoclonal, Recombinant

• Clone:

  A762

• Conjugation:

  Unconjugated

• Applications:

  WB

• Field of Research:

  Neuroscience; Epigenetics and Nuclear Signaling; Cancer

• Purification:

  Protein A purified

• Concentration:

  1 mg/mL

• Dilution:

  WB 1:2000

• Buffer:

  PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  73 kDa

• Observed Molecular Weight:

  70 kDa

• Isotype:

  IgG, κ