Recombinant ALIX Monoclonal Antibody

• Background:

  Programmed cell death 6-interacting protein (PDCD6IP), plays an important role in the endosomal sorting complexes required for transport (ESCRT) pathway in the abscission stage of cytokinesis and apoptosis. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition, the protein binds to the product of the PDCD6 gene, a protein required for apoptosis, in a calcium-dependent manner. It also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of this protein and endophilins results in cytoplasmic vacuolization, which may be partly responsible for the protection against cell death.

• Abbreviation:

  ALIX

• UniProt:

  Q8WUM4

• Host:

  Rabbit

• Reactivity:

  Human; Rat; Mouse

• Immunogen:

  Recombinant human ALIX fragment

• Target:

  AIP; DRIP; KIAA; PDCD6IP; AIP1; ALIX; DRIP4; HP95; KIAA1375

• Clonality:

  Monoclonal, Recombinant

• Clone:

  A493

• Conjugation:

  Unconjugated

• Applications:

  WB; IHC; IP

• Field of Research:

  Cell Biology; Microbiology; Signal Transduction

• Purification:

  Protein A purified

• Concentration:

  1 mg/mL

• Dilution:

  WB 1:2000-1:10000; IHC 1:100-1:500; IP 1:25-1:50

• Buffer:

  PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  96 kDa

• Observed Molecular Weight:

  90/100 kDa

• Isotype:

  IgG, κ