TIE2 (phospho Tyr992) Antibody

• Background:

  The TEK receptor tyrosine kinase is expressed almost exclusively in endothelial cells in mice, rats, and humans. This receptor possesses a unique extracellular domain containing 2 immunoglobulin-like loops separated by 3 epidermal growth factor-like repeats that are connected to 3 fibronectin type III-like repeats. The ligand for the receptor is angiopoietin-1. Defects in TEK are associated with inherited venous malformations; the TEK signaling pathway appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis. TEK is closely related to the TIE receptor tyrosine kinase.

• NCBI Gene ID:

  7010

• Swiss Prot:

  Q02763

• Accession Number:

  NP_000450.2

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  TIE2 (phospho Tyr992) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 992 (E-V-Y (p) -V-K) derived from Human TIE2.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Phospho-Specific

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific peptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  Phospho-Specific

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  126 kDa

• Fragment:

  IgG

• Specificity:

  The Antibody detects endogenous levels of TIE2 only when phosphorylated at Tyr992.

• Applications Notes:

  Western Blot: 1:500~1:1000

• Symbol:

  TEK

• NCBI Official Name:

  TEK tyrosine kinase, endothelial

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Schooley K.A., Baum P.R. Oncogene 8:663-670 (1993)

• Background Reference 02:

  Dunham I. Nature 429:369-374 (2004)

• Background Reference 03:

  Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases

• Other Product Names:

  Angiopoietin-1 receptor, EC=2.7.10.1 Tunica interna endothelial cell kinase Tyrosine-protein kinase receptor TEK Tyrosine-protein kinase receptor TIE-2 hTIE2 p140 TEK CD202b TEK TIE2

• Tested Applications:

  WB

• Protein ID:

  218511853

• Physical Properties:

  Liquid