14 3 3 zeta (Ab 58) Antibody

• Background:

  This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene.

• NCBI Gene ID:

  7534

• Swiss Prot:

  P63104

• Accession Number:

  NP_001129171.1

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  14-3-3 zeta (Ab-58) antibody was raised against a peptide sequence around aa.56~60 (R-S-S-W-R) derived from Human 14-3-3 zeta.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Apoptosis, Cancer, Cell Cycle

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific peptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  28 kDa

• Specificity:

  This antibody detects endogenous level of total 14-3-3 zeta protein.

• Applications Notes:

  Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

• Symbol:

  YWHAZ

• NCBI Official Name:

  Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Gu YM, et al. (2006) FEBS Lett ; 580 (1) : 305-310Powell DW, et al. (2003) Mol Cell Biol; 23 (15) : 5376-5387Mackintosh C. (2004) Biochem J; 381 (Pt 2) : 329-342.

• Other Product Names:

  HEL4, YWHAD, KCIP-1, HEL-S-3, 14-3-3-zeta, ERBB, ERBB1, HER1, 1433Z, 143Z, FAS, Factor activating exoenzyme S

• Tested Applications:

  WB, IHC

• Protein ID:

  208973238

• Physical Properties:

  Liquid