Smad3 (phospho Ser425) Antibody

• Background:

  Smad3 encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.

• NCBI Gene ID:

  4088

• Swiss Prot:

  P84022

• Accession Number:

  NP_001138574.1

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Smad3 (Phospho-Ser425) antibody was raised against a peptide sequence around phosphorylation site of serine 425 (C-S-S-V-S (p) ) derived from Human Smad3.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Phospho-Specific

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  Phospho-Specific

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  52 kDa

• Specificity:

  This antibody detects endogenous level of Smad3 only when phosphorylated at serine 425.

• Applications Notes:

  Immunohistochemistry: 1:50~1:100

• Symbol:

  SMAD3

• NCBI Official Name:

  SMAD family member 3

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Shi W, et al. J Cell Sci. 2007 Apr 1;120 (Pt 7) : 1216-24Seong HA, et al. J Biol Chem. 2007 Apr 20;282 (16) : 12272-89Wordinger RJ, et al. Invest Ophthalmol Vis Sci. 2007 Mar;48 (3) : 1191-200LeClair RJ, et al. Circ Res. 2007 Mar 30;100 (6) : 826-33

• Other Product Names:

  LDS3, LDS1C, MADH3, JV15-2, HSPC193, HsT17436, PTP2C, SHPTP2, MAD-3, Mad3, Mothers against DPP homolog 3

• Tested Applications:

  IHC

• Protein ID:

  223029440

• Physical Properties:

  Liquid