Progesterone Receptor (phospho Ser190) Antibody

• Background:

  Progesterone receptors (PRs) are nuclear hormone receptors of the NR3C class, which also includes mineralocorticoid, glucocorticoid and androgen receptors. They exist as homodimers coupled to Hsp90 or HMGB proteins, which are shed upon activation. The major signaling pathway used by progesterone receptors is via direct DNA binding and transcriptional regulation of target genes. They can also signal by binding to other proteins, mainly with transcription factors such as NF-κ B, AP-1 or STAT. Progesterone receptors are found in the female reproductive tract, mammary glands, brain and pituitary gland and receptor expression is induced by estrogen. Well established functions of progesterone receptors include ovulation, implantation, mammary gland development and maintenance of pregnancy. In addition, progesterone, signaling through the progesterone receptor, increases the ventilatory response of the respiratory centers to carbon dioxide and decreases arterial and alveolar PCO2 in the luteal phase of the menstrual cycle and during pregnancy. The human gene encoding the progesterone receptor has been localized to 11q22.

• NCBI Gene ID:

  5241

• Swiss Prot:

  P06401

• Accession Number:

  NP_000917.3

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Progesterone Receptor (Phospho-Ser190) antibody was raised against a peptide sequence around phosphorylation site of serine 190 (G-L-S (p) -P-A) derived from Human Progesterone Receptor.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Phospho-Specific

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  Phospho-Specific

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  99 kDa

• Specificity:

  This antibody detects endogenous level of Progesterone Receptor only when phosphorylated at serine 190.

• Applications Notes:

  Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

• Symbol:

  PGR

• NCBI Official Name:

  Progesterone receptor

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Narayanan R, et al. (2005) Mol Cell Biol; 25 (8) : 2885-98. Knotts TA, et al. (2001) J Biol Chem; 276 (11) : 8475-83. Clemm DL, et al. (2000) Mol Endocrinol; 14 (1) : 52-65. Zhang Y, et al. (1997) Mol Endocrinol; 11 (6) : 823-32

• Other Product Names:

  PR, NR3C3, IL10R, PGR, PRGR

• Tested Applications:

  WB, IHC, IF

• Protein ID:

  110611914

• Physical Properties:

  Liquid