ATF2 (phospho Ser62 / 44) Antibody

• Background:

  Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2-c-Jun complex.

• NCBI Gene ID:

  1386

• Swiss Prot:

  P15336

• Accession Number:

  NP_001871.2

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  ATF2 (Phospho-Ser62 or 44) antibody was raised against a peptide sequence around phosphorylation site of serine 62 or 44 (N-D-S (p) -V-I) derived from Human ATF2.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Phospho-Specific

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  Phospho-Specific

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  65 - 75 kDa

• Specificity:

  This antibody detects endogenous level of ATF-2 only when phosphorylated at serine 62 or 44.

• Applications Notes:

  Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

• Symbol:

  ATF2

• NCBI Official Name:

  Activating transcription factor 2

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Sevilla A, et al. (2004) J Biol Chem. 279 (26) : 27458-27465. Sakurai A, et al. (1991) Biochem Biophys Res Commun. 181 (2) : 629-635. Abdel-Hafiz H A, et al. (1992) Mol Endocrinol. 6: 2079-2089. Gupta S, et al. (1995) Science. 267: 389-393. Van Dam H, et al. (1995) EMBO J. 14 (8) : 1798-1811.

• Other Product Names:

  HB16, CREB2, TREB7, CREB-2, CRE-BP1, MADH3, CREBP1

• Tested Applications:

  WB, IHC

• Protein ID:

  22538422

• Physical Properties:

  Liquid