Connexin 40 Antibody

• Background:

  Gap junctions were first characterized by electron microscopy as regionally specialized structures on plasma membranes of contacting adherent cells. These structures were shown to consist of cell-to-cell closely packed transmembrane channels. Proteins, called connexins, purified from fractions of enriched gap junctions from different tissues differ. Connexins are designated by their molecular mass. Another system of nomenclature divides gap junction proteins into 2 categories, alpha and beta, according to sequence similarities at the nucleotide and amino acid levels. For example, CX43 is designated alpha-1 gap junction protein, whereas CX32 and CX26 are called beta-1 and beta-2 gap junction proteins, respectively. This nomenclature emphasizes that CX32 and CX26 are more homologous to each other than either of them is to CX43. Connexins have four transmembrane, three intracellular, and two extracellular regions. Different tissues express different connexins, though tissue specificities overlap, and a given tissue or cell can express several different connexins. Developmental regulation of at least some of the connexin genes has been found. Embryo implantation is regulated in part by temporally changing patterns of expression of connexins in the embryo and the maternal decidua.

• NCBI Gene ID:

  2702

• Swiss Prot:

  P36382

• Accession Number:

  P36382

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This Connexin 40 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 102-134 amino acids from the N-terminal region of human Connexin 40.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Signal Transduction

• Purification:

  This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  40 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  GJA5

• NCBI Official Name:

  Gap junction alpha-5 protein

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Dupays, L., et al., Gene 305 (1) :79-90 (2003) .

• Background Reference 02:

  Simon, A.M., et al., Dev. Biol. 251 (2) :206-220 (2002) .

• Background Reference 03:

  Cronier, L., et al., Mol. Hum. Reprod. 8 (11) :1005-1013 (2002) .

• Background Reference 04:

  Oviedo-Orta, E., et al., Immunology 99 (4) :578-590 (2000) .

• Other Product Names:

  Gap junction alpha-5 protein, Connexin-40, Cx40, GJA5

• Tested Applications:

  WB, IHC-P

• Protein ID:

  8928556

• Physical Properties:

  Liquid